分析两栖动物中促黑素细胞激素的辐射：从有尾两栖动物大鳗螈和斑泥螈的垂体中克隆阿黑皮素原cDNA。

Analyzing the radiation of the melanocortins in amphibians: cloning of POMC cDNAs from the pituitary of the urodele amphibians, Amphiuma means and Necturus maculosus.

作者信息

Kozak Katarzyna, Costantino David, Lecaude Stephanie, Sollars Cristina, Danielson Phillip, Dores Robert M

机构信息

University of Denver, Department of Biological Sciences, Denver, CO 80210, USA.

出版信息

Peptides. 2005 Oct;26(10):1920-8. doi: 10.1016/j.peptides.2004.12.025.

DOI:10.1016/j.peptides.2004.12.025

PMID:16005111

Abstract

Proopiomelanocortin (POMC) cDNAs were cloned and sequenced from brain extracts of two species of urodele amphibians: Amphiuma means and Necturus maculosus. Although the two species of urodele amphibians belong to separate families, and do not share a direct common ancestor, the level of primary sequence identity for the open reading of the POMC cDNAs was 90% at the amino acid level and 79% at the nucleotide level. It appears that the POMC gene in these urodele amphibians has been accumulating mutations at the amino acid level at a slower rate than the POMC gene in other sarcopterygian orders.

摘要

促阿片-促黑素细胞皮质素原（POMC）的互补脱氧核糖核酸（cDNA）是从两种有尾两栖动物——美洲鳗螈和斑泥螈的脑提取物中克隆并测序得到的。尽管这两种有尾两栖动物属于不同的科，且没有直接的共同祖先，但POMC cDNA开放阅读框的一级序列在氨基酸水平上的同一性为90%，在核苷酸水平上为79%。看来，这些有尾两栖动物中的POMC基因在氨基酸水平上积累突变的速度比其他肉鳍鱼类目中的POMC基因要慢。