流动注射分光光度法测定药物和尿液中的氧氟沙星。（注：原英文文本中多了一个“of”，正确的应该是“Flow injection spectrophotometric determination of ofloxacin in pharmaceuticals and urine.” ）

Flow injection spectrophotometric determination of ofloxacin in pharmaceuticals and urine.

作者信息

Soledad García M, Isabel Albero M, Sánchez-Pedreño Concepción, Salem Abuherba Mustafa

机构信息

Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Murcia, Spain.

出版信息

Eur J Pharm Biopharm. 2005 Sep;61(1-2):87-93. doi: 10.1016/j.ejpb.2005.03.007.

DOI:10.1016/j.ejpb.2005.03.007

PMID:16005199

Abstract

A sensitive and fast flow-injection spectrophotometric method for the determination of ofloxacin is proposed. The method is based on the formation of a yellow complex between ofloxacin and Fe (III), in sulphuric medium. The influence of FI and chemical variables were studied. The calibration graph resulting from measuring the absorbance at 420 nm is linear over the range 5.0 x 10(-6)8.0 x 10(-4)M (1.8-289 mg l(-1)) with a detection limit of 2.0 x 10(-6)M (0.72 mg l(-1)). The method is applied to the routine analysis of ofloxacin in pharmaceuticals and human urine.

摘要

提出了一种灵敏、快速的流动注射分光光度法测定氧氟沙星。该方法基于在硫酸介质中氧氟沙星与铁（III）形成黄色络合物。研究了流动注射和化学变量的影响。在420nm处测量吸光度得到的校准曲线在5.0×10⁻⁶至8.0×10⁻⁴M（1.8 - 289mg l⁻¹）范围内呈线性，检测限为2.0×10⁻⁶M（0.72mg l⁻¹）。该方法应用于药物和人尿中氧氟沙星的常规分析。