Zhao Shulin, Yuan Hongyan, Xiao Dan
College of Chemical Engineering, Sichuan University, Chengdu 610065, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 5;822(1-2):334-8. doi: 10.1016/j.jchromb.2005.06.027.
A capillary zone electrophoresis method with laser induced fluorescence detection for the chiral separation of highly fluorescent enantiomeric derivatives of d/l-Serine from 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-d/l-Serine) was developed and optimized. Enantiomeric separation of NBD-d/l-Serine was accomplished by using 40 mM hydroxypropyl-beta-cyclodextrin (HP-beta-CD) contained in 100 mM borate buffer, pH 10.0. A 70 cm (effective length of 50 cm) uncoated fused-silica capillary at a voltage of 15 kV was used for the separation. The optimized electrophoretic conditions were subsequently applied to the analysis of d-Serine in rat brain, and satisfactory analytical results with respect to accuracy were obtained. This assay showed acceptable precision, with linearity in the d-Serine concentration range of 0.2-20.0 microM. The limit of detection for d-Serine was 3.0 x 10(-7)M.
建立并优化了一种采用激光诱导荧光检测的毛细管区带电泳方法，用于手性分离4-氟-7-硝基-2,1,3-苯并恶二唑衍生的高荧光对映体d/l-丝氨酸（NBD-d/l-丝氨酸）。通过使用100 mM硼酸盐缓冲液（pH 10.0）中含有的40 mM羟丙基-β-环糊精（HP-β-CD）实现了NBD-d/l-丝氨酸的对映体分离。使用一根70 cm（有效长度50 cm）未涂层熔融石英毛细管，在15 kV电压下进行分离。随后将优化后的电泳条件应用于大鼠脑中d-丝氨酸的分析，并获得了关于准确性的满意分析结果。该测定法显示出可接受的精密度，d-丝氨酸浓度范围在0.2 - 20.0 microM时具有线性关系。d-丝氨酸的检测限为3.0×10⁻⁷M。
