Annerberg A, Singtoroj T, Tipmanee P, White N J, Day N P J, Lindegårdh N
Wellcome unit, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Aug 5;822(1-2):330-3. doi: 10.1016/j.jchromb.2005.06.022.
A high throughput bioanalytical assay for the determination of lumefantrine in plasma has been developed and validated extensively. The within-day precisions for lumefantrine were 5.2, 3.5 and 2.5% at 200, 2000 and 15000 ng/mL, respectively. The between-day precisions were 4.0, 2.8 and 3.1% at 200, 2000 and 15000 ng/mL, respectively. The lower limits of quantification (LLOQ) and the limits of detection (LOD) were 25 and 10 ng/mL, respectively using 0.250 mL plasma. The average recovery of lumefantrine was 85% and independent upon concentration. The use of 96-well plate format and short chromatographic run has increased the daily sample throughput four times. The assay is particularly suitable for large therapeutic drug monitoring studies using day 7 sampling.
已开发并广泛验证了一种用于测定血浆中卤泛群的高通量生物分析方法。卤泛群的日内精密度在200、2000和15000 ng/mL时分别为5.2%、3.5%和2.5%。日间精密度在200、2000和15000 ng/mL时分别为4.0%、2.8%和3.1%。使用0.250 mL血浆时，定量下限（LLOQ）和检测限（LOD）分别为25和10 ng/mL。卤泛群的平均回收率为85%，且与浓度无关。96孔板形式的使用和较短的色谱运行时间使每日样品通量提高了四倍。该方法特别适用于使用第7天采样的大型治疗药物监测研究。
