Wang Yan, Zhao Kan-xing, Su Lei-yan, Wang Le-jin, Geng Wei-li, Jin Ying
Tianjin Eye Hospital, Tianjin 300020, China.
Zhonghua Yan Ke Za Zhi. 2005 Jun;41(6):498-504.
To assess the effects of 20% ethanol used in LASEK on corneal wound healing.
Forty-eight eyes from 24 rabbits were deepithelialized by two techniques. The epithelium were detached with either 20% ethanol (applied for 30 seconds) or mechanical scraping, then ablated was performed. The number of superficial stromal keratocytes was counted and the morphologic changes were observed. Expression of TGF-beta1 and bFGF mRNAs was detected and analyzed by reverse transcription-polymerase chain reaction and immunocytochemical methods at 1, 7, 30 and 90 days after the surgery.
One day after the surgery, the expression of TGF-beta1 and bFGF in the keratocytes in both treated groups was lower than that of the normal controls. Seven days after the surgery, the expression of TGF-beta1 and bFGF in both treated groups was greater than that of the normal controls (P < 0.01). The expression in the alcohol-treated group was greater than that of the surgical-treated group (P < 0.01). The expression of TGF-beta1 and bFGF reached the peak 30 days after the surgery, no significant difference was detected between the alcohol-treated and surgical-treated groups. There was no significant difference in expression level between the alcohol-treated and surgical-treated groups 3 months after the surgery; as well as between treated groups and normal group. The amount of TGF-beta1 and bFGF mRNA was positively correlated with the number of keratocytes. The correlation coefficient between TGF-beta1 and bFGF mRNA and the number of the keratocytes was 0.744 (P < 0.01) and 0.738 (P < 0.01) in the alcohol-treated group; and was 0.664 (P < 0.01) and 0.785 (P < 0.01) in the surgical-treated group, respectively.
The expression of TGF-beta1 and bFGF mRNA undergo a dynamic process of "decrease-increase-normal". Although ethanol has a slight toxic effect on rabbit epithelial cells, but the effects do not persist over time, therefore, it is relatively safe to use the alcohol treatment in the LASEK.
评估准分子激光上皮下角膜磨镶术（LASEK）中使用的20%乙醇对角膜伤口愈合的影响。
采用两种技术对24只兔子的48只眼进行上皮去除。用20%乙醇（应用30秒）或机械刮除分离上皮，然后进行消融。计数浅表基质角膜细胞数量并观察形态学变化。在手术后1、7、30和90天，通过逆转录-聚合酶链反应和免疫细胞化学方法检测并分析转化生长因子-β1（TGF-β1）和碱性成纤维细胞生长因子（bFGF）mRNA的表达。
手术后1天，两个治疗组角膜细胞中TGF-β1和bFGF的表达均低于正常对照组。手术后7天，两个治疗组中TGF-β1和bFGF的表达均高于正常对照组（P<0.01）。乙醇治疗组的表达高于手术治疗组（P<0.01）。TGF-β1和bFGF的表达在手术后30天达到峰值，乙醇治疗组和手术治疗组之间未检测到显著差异。手术后3个月，乙醇治疗组和手术治疗组之间的表达水平无显著差异；治疗组与正常组之间也无显著差异。TGF-β1和bFGF mRNA的量与角膜细胞数量呈正相关。在乙醇治疗组中，TGF-β1和bFGF mRNA与角膜细胞数量的相关系数分别为0.744（P<0.01）和0.738（P<0.01）；在手术治疗组中分别为0.664（P<0.01）和0.785（P<0.01）。
TGF-β1和bFGF mRNA的表达经历“降低-升高-正常”的动态过程。虽然乙醇对兔上皮细胞有轻微毒性作用，但这种作用不会随时间持续存在，因此，在LASEK中使用乙醇治疗相对安全。
