Liu Gang, Nakayama Kazuko, Sagara Yasuhiro, Awata Shiro, Yamashita Koichi, Manabe Masanobu, Kodama Hiroyuki
Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Oko-cho, Nankoku-shi, Kochi 783-8505, Japan.
Clin Biochem. 2005 Jul;38(7):625-31. doi: 10.1016/j.clinbiochem.2005.03.007.
The purpose of this study was to investigate the effect of various amino acids and their metabolites on the activities of prolidase I and II from human erythrocytes compared to those in a patient with prolidase deficiency.
Prolidase I and II from human erythrocytes were purified by using column chromatography. Prolidase activity against various iminodipeptides was determined by spectrophotometry using Chinard's method.
The activities of prolidase I and II against glycylproline and methionylproline were enhanced by glycine, L- and D-isoforms of alanine and serine and D-isoforms of valine, leucine and isoleucine. L-isoforms of branched amino acids inhibited the activity of prolidase I. On the other hand, the activity of prolidase II was enhanced by all of these L-branched amino acids. The patient's prolidase activity was also enhanced by all the L- and D-branched amino acids.
The activities of prolidase I and II against various iminodipeptides were prominently enhanced by glycine, but the effect of L-valine differed between the two enzymes. Enzymatic properties of the patient's prolidase were essentially the same as those of prolidase II.
本研究旨在调查与脯氨肽酶缺乏症患者相比，各种氨基酸及其代谢产物对人红细胞中脯氨肽酶I和II活性的影响。
采用柱色谱法纯化人红细胞中的脯氨肽酶I和II。使用Chinard法通过分光光度法测定针对各种亚氨基二肽的脯氨肽酶活性。
甘氨酸、丙氨酸和丝氨酸的L型和D型异构体以及缬氨酸、亮氨酸和异亮氨酸的D型异构体可增强脯氨肽酶I和II对甘氨酰脯氨酸和甲硫氨酰脯氨酸的活性。支链氨基酸的L型异构体抑制脯氨肽酶I的活性。另一方面，所有这些L型支链氨基酸均可增强脯氨肽酶II的活性。所有L型和D型支链氨基酸也可增强患者的脯氨肽酶活性。
甘氨酸可显著增强脯氨肽酶I和II对各种亚氨基二肽的活性，但L型缬氨酸对这两种酶的作用有所不同。患者脯氨肽酶 的酶学性质与脯氨肽酶II基本相同。
