鉴定酵母沉默交配位点抑制所需的组蛋白H4 N端非碱性结构域。
Identification of a non-basic domain in the histone H4 N-terminus required for repression of the yeast silent mating loci.
作者信息
Johnson L M, Fisher-Adams G, Grunstein M
机构信息
Molecular Biology Institute, University of California, Los Angeles 90024.
出版信息
EMBO J. 1992 Jun;11(6):2201-9. doi: 10.1002/j.1460-2075.1992.tb05279.x.
Abstract
We have shown previously that a stretch of four charged residues (16-19) at the histone H4 N-terminus is involved in repression of the yeast silent mating loci. One of these residues, Lys16, is a site for acetylation, which may prevent repression of the silent mating loci. In this paper we ask whether other sequences in histone H4, possibly in conjunction with H3 residues, are required for repression. We find that even in combination, the other seven acetylatable lysines in H3 and H4 do not function in repression. In contrast, we have found that an adjacent relatively uncharged domain (residues 21-29) is required for repression and that single amino acid insertions and deletions in this region are extremely detrimental. We propose that the basic and non-basic domains together form a DNA (or protein) induced amphipathic alpha-helix required in the formation of a repressive chromatin structure.
摘要
我们之前已经表明，组蛋白H4 N端的一段四个带电荷的残基（16 - 19）参与了酵母沉默交配位点的抑制。其中一个残基，赖氨酸16，是乙酰化位点，这可能会阻止沉默交配位点的抑制。在本文中，我们探讨组蛋白H4中的其他序列，可能与H3残基一起，是否是抑制所必需的。我们发现，即使结合起来，H3和H4中的其他七个可乙酰化赖氨酸在抑制中也不起作用。相反，我们发现一个相邻的相对不带电荷的结构域（残基21 - 29）是抑制所必需的，并且该区域中的单个氨基酸插入和缺失极其有害。我们提出，碱性和非碱性结构域共同形成一个DNA（或蛋白质）诱导的两亲性α-螺旋，这是形成抑制性染色质结构所必需的。
相似文献
1
Identification of a non-basic domain in the histone H4 N-terminus required for repression of the yeast silent mating loci.鉴定酵母沉默交配位点抑制所需的组蛋白H4 N端非碱性结构域。
EMBO J. 1992 Jun;11(6):2201-9. doi: 10.1002/j.1460-2075.1992.tb05279.x.
2
Histone H3 amino terminus is required for telomeric and silent mating locus repression in yeast.组蛋白H3的氨基末端是酵母中端粒和沉默交配位点抑制所必需的。
Nature. 1994 May 19;369(6477):245-7. doi: 10.1038/369245a0.
3
Genetic evidence for an interaction between SIR3 and histone H4 in the repression of the silent mating loci in Saccharomyces cerevisiae.酿酒酵母中SIR3与组蛋白H4在沉默交配型基因座抑制过程中相互作用的遗传学证据。
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6286-90. doi: 10.1073/pnas.87.16.6286.
4
Histone H3 N-terminal mutations allow hyperactivation of the yeast GAL1 gene in vivo.组蛋白H3的N端突变可在体内使酵母GAL1基因过度激活。
EMBO J. 1992 Sep;11(9):3297-306. doi: 10.1002/j.1460-2075.1992.tb05408.x.
5
Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern.酿酒酵母中的高效转录沉默需要异染色质组蛋白乙酰化模式。
Mol Cell Biol. 1996 Aug;16(8):4349-56. doi: 10.1128/MCB.16.8.4349.
6
Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML.酵母组蛋白H4基因中的点突变会阻止沉默交配型位点HML的沉默。
Mol Cell Biol. 1990 Sep;10(9):4932-4. doi: 10.1128/mcb.10.9.4932-4934.1990.
7
Genome-wide analysis of the relationship between transcriptional regulation by Rpd3p and the histone H3 and H4 amino termini in budding yeast.芽殖酵母中Rpd3p介导的转录调控与组蛋白H3和H4氨基末端之间关系的全基因组分析。
Mol Cell Biol. 2004 Oct;24(20):8823-33. doi: 10.1128/MCB.24.20.8823-8833.2004.
8
Redundant roles for histone H3 N-terminal lysine residues in subtelomeric gene repression in Saccharomyces cerevisiae.组蛋白H3 N端赖氨酸残基在酿酒酵母亚端粒基因抑制中的多重作用
Genetics. 2004 Jul;167(3):1123-32. doi: 10.1534/genetics.104.026674.
9
Deciphering the roles of the histone H2B N-terminal domain in genome-wide transcription.解析组蛋白H2B N端结构域在全基因组转录中的作用。
Mol Cell Biol. 2006 May;26(10):3842-52. doi: 10.1128/MCB.26.10.3842-3852.2006.
10
Amino termini of histones H3 and H4 are required for a1-alpha2 repression in yeast.酵母中α1-α2抑制需要组蛋白H3和H4的氨基末端。
Mol Cell Biol. 1997 Nov;17(11):6555-62. doi: 10.1128/MCB.17.11.6555.
引用本文的文献
1
Highly specific intracellular ubiquitination of a small molecule.一种小分子的高度特异性细胞内泛素化作用。
Nat Chem Biol. 2025 Aug 21. doi: 10.1038/s41589-025-02011-1.
2
Highly specific intracellular ubiquitination of a small molecule.一种小分子的高度特异性细胞内泛素化作用。
bioRxiv. 2024 Jan 28:2024.01.26.577493. doi: 10.1101/2024.01.26.577493.
3
Regulation of the heterochromatin spreading reaction by acting factors.作用因子对异染色质扩散反应的调控。
Open Biol. 2023 Nov;13(11):230271. doi: 10.1098/rsob.230271. Epub 2023 Nov 8.
4
Measuring the buffering capacity of gene silencing in .测量基因沉默的缓冲能力。
Proc Natl Acad Sci U S A. 2021 Dec 7;118(49). doi: 10.1073/pnas.2111841118.
5
The many faces of histone H3K79 methylation.组蛋白 H3K79 甲基化的多面性。
Mutat Res Rev Mutat Res. 2016 Apr-Jun;768:46-52. doi: 10.1016/j.mrrev.2016.03.005. Epub 2016 Mar 31.
6
Interpreting the language of histone and DNA modifications.解读组蛋白和DNA修饰的语言。
Biochim Biophys Acta. 2014 Aug;1839(8):627-43. doi: 10.1016/j.bbagrm.2014.03.001. Epub 2014 Mar 12.
7
The histone H4 tail regulates the conformation of the ATP-binding pocket in the SNF2h chromatin remodeling enzyme.组蛋白 H4 尾部调节 SNF2h 染色质重塑酶中 ATP 结合口袋的构象。
J Mol Biol. 2014 May 15;426(10):2034-44. doi: 10.1016/j.jmb.2014.02.021. Epub 2014 Mar 4.
8
The double PHD finger domain of MOZ/MYST3 induces α-helical structure of the histone H3 tail to facilitate acetylation and methylation sampling and modification.MOZ/MYST3 的双 PHD 指结构域诱导组蛋白 H3 尾部形成α-螺旋结构，从而促进乙酰化和甲基化的采样和修饰。
Nucleic Acids Res. 2014 Jan;42(2):822-35. doi: 10.1093/nar/gkt931. Epub 2013 Oct 22.
9
The role of multiple marks in epigenetic silencing and the emergence of a stable bivalent chromatin state.多个标记在表观遗传沉默中的作用以及稳定的双价染色质状态的出现。
PLoS Comput Biol. 2013;9(7):e1003121. doi: 10.1371/journal.pcbi.1003121. Epub 2013 Jul 18.
10
Epigenetics in Saccharomyces cerevisiae.酿酒酵母中的表观遗传学。
Cold Spring Harb Perspect Biol. 2013 Jul 1;5(7):a017491. doi: 10.1101/cshperspect.a017491.
本文引用的文献
1
Orientation of the nucleosome within the higher order structure of chromatin.核小体在染色质高级结构中的定位。
Cell. 1980 Nov;22(1 Pt 1):87-96. doi: 10.1016/0092-8674(80)90157-9.
2
Acetylation of histone H4 and its role in chromatin structure and function.组蛋白H4的乙酰化及其在染色质结构和功能中的作用。
Nature. 1980 Sep 4;287(5777):76-9. doi: 10.1038/287076a0.
3
Effect of acetylation on the binding of N-terminal peptides of histone H4 to DNA.乙酰化对组蛋白H4 N端肽与DNA结合的影响。
Eur J Biochem. 1982 Sep;127(1):137-43. doi: 10.1111/j.1432-1033.1982.tb06847.x.
4
Participation of core histone "tails" in the stabilization of the chromatin solenoid.核心组蛋白“尾巴”在染色质螺线管稳定中的作用。
J Cell Biol. 1982 May;93(2):285-97. doi: 10.1083/jcb.93.2.285.
5
Role of DNA replication in the repression of silent mating type loci in yeast.DNA复制在酵母沉默交配型基因座抑制中的作用。
Nature. 1984;312(5991):247-51. doi: 10.1038/312247a0.
6
A position effect in the control of transcription at yeast mating type loci.酵母交配型基因座转录调控中的位置效应。
Nature. 1981 Jan 22;289(5795):244-50. doi: 10.1038/289244a0.
7
The regulation of yeast mating-type chromatin structure by SIR: an action at a distance affecting both transcription and transposition.SIR对酵母交配型染色质结构的调控：远距离作用对转录和转座的影响
Cell. 1982 Sep;30(2):567-78. doi: 10.1016/0092-8674(82)90253-7.
8
Identification of sites required for repression of a silent mating type locus in yeast.酵母中沉默交配型基因座抑制所需位点的鉴定
J Mol Biol. 1984 Oct 5;178(4):815-34. doi: 10.1016/0022-2836(84)90313-9.
9
Simple computer-aided approach for the analyses of the nuclear-magnetic-resonance spectra of histones. Fractions F1, Fsa1, F2B, cleaved halves of F2B and F2B-DNA.用于分析组蛋白核磁共振谱的简单计算机辅助方法。组分F1、Fsa1、F2B、F2B的裂解片段以及F2B-DNA。
Eur J Biochem. 1972 May 23;27(2):270-81. doi: 10.1111/j.1432-1033.1972.tb01836.x.
10
The ARD1 gene of yeast functions in the switch between the mitotic cell cycle and alternative developmental pathways.
Cell. 1985 Dec;43(2 Pt 1):483-92. doi: 10.1016/0092-8674(85)90178-3.
Zotero 插件