Babu Gopal J, Periasamy Muthu
Department of Physiology and Cell Biology, The Ohio State University, Columbus, OH, USA.
Methods Mol Med. 2005;112:365-77. doi: 10.1385/1-59259-879-x:365.
The sarcoplasmic reticulum Ca2+ ATPase (SERCA) plays a pivotal role in calcium cycling and the beat-to-beat function of the heart. Recent studies have shown that decreased expression and activity of SERCA are associated with end-stage heart failure in humans and in experimental animal models of heart failure. There has been considerable controversy over whether a decrease in SERCA level is a cause or effect of hypertrophy. To address directly whether alterations in SERCA levels modify calcium homeostasis and heart function, we have chosen to alter the SERCA protein expression genetically using transgenic and gene-targeted knockout mouse technology. This chapter describes the methodology for generation of mouse models that overexpress different SERCA isoforms and a SERCA2 knockout mouse model with decreased SERCA levels.
肌浆网Ca2+ATP酶（SERCA）在钙循环及心脏逐搏功能中起关键作用。最近的研究表明，SERCA表达及活性降低与人类终末期心力衰竭以及心力衰竭实验动物模型相关。关于SERCA水平降低是肥大的原因还是结果一直存在很大争议。为了直接探讨SERCA水平改变是否会影响钙稳态及心脏功能，我们选择利用转基因和基因靶向敲除小鼠技术从基因层面改变SERCA蛋白表达。本章介绍了生成过表达不同SERCA亚型的小鼠模型以及SERCA水平降低的SERCA2敲除小鼠模型的方法。
