Shimizu Daisuke, Ishikawa Takashi, Ichikawa Yasushi, Togo Shinji, Hayasizaki Yoshihide, Okazaki Yasushi, Danenberg Peter V, Shimada Hiroshi
Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa 236-0004, Japan.
Int J Oncol. 2005 Aug;27(2):371-6.
5-Fluorouracil (5-FU) is the most widely used anticancer agent for gastrointestinal cancers. Because many tumors show primary resistance, it is clinically meaningful to predict tumor sensitivity to the drug before treatment. cDNA microarrays containing 21,168 clones were used to identify genes associated with sensitivity to 5-FU. Gene expression profiling of 3 colorectal cancer cell lines (DLD-1, HT-29 and NUGC-3) and the corresponding 5-FU-resistant sublines (DLD-1/FU, HT-29/FU and NUGC-3/5FU/L) showed 81 genes that were differentially expressed. The gene set thus identified successfully predicted the sensitivities of 5 other colorectal cancer cell lines and could also separate 5-FU resistant clinical samples from sensitive ones.
5-氟尿嘧啶（5-FU）是治疗胃肠道癌症最常用的抗癌药物。由于许多肿瘤表现出原发性耐药，因此在治疗前预测肿瘤对该药物的敏感性具有临床意义。使用包含21,168个克隆的cDNA微阵列来鉴定与对5-FU敏感性相关的基因。对3种结肠癌细胞系（DLD-1、HT-29和NUGC-3）以及相应的5-FU耐药亚系（DLD-1/FU、HT-29/FU和NUGC-3/5FU/L）进行基因表达谱分析，结果显示有81个基因存在差异表达。由此鉴定出的基因集成功预测了其他5种结肠癌细胞系的敏感性，还能将5-FU耐药的临床样本与敏感样本区分开来。
