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Reactivation of Rickettsia rickettsii in Dermacentor andersoni ticks: an ultrastructural analysis.安德森革蜱中里氏立克次体的再激活:超微结构分析
Infect Immun. 1982 Aug;37(2):779-85. doi: 10.1128/iai.37.2.779-785.1982.
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Serotypes of spotted fever group rickettsiae isolated from Dermacentor andersoni (Stiles) ticks in western Montana.从蒙大拿州西部的安氏革蜱(斯泰尔斯)中分离出的斑点热群立克次体血清型。
Am J Trop Med Hyg. 1981 Jan;30(1):230-8. doi: 10.4269/ajtmh.1981.30.230.
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Resurgence of Mediterranean spotted fever in Spain.
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Canine Rocky Mountain spotted fever: a kennel epizootic.
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First isolates of Rickettsia conorii in Spain using a centrifugation-shell vial assay.
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7
Reactivity of monoclonal antibodies to Rickettsia rickettsii with spotted fever and typhus group rickettsiae.抗立氏立克次体单克隆抗体与斑点热群和斑疹伤寒群立克次体的反应性。
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从西班牙马德里的蜱卵中分离出斑点热群立克次体病原体。

Isolation of an agent of the spotted fever group rickettsia from tick eggs in Madrid, Spain.

作者信息

Herrero C, Pelaz C, Martín-Bourgon C

机构信息

Servicio de Bacteriología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

出版信息

Epidemiol Infect. 1992 Jun;108(3):555-7. doi: 10.1017/s0950268800050056.

DOI:10.1017/s0950268800050056
PMID:1601085
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2272212/
Abstract

Ticks recovered from dogs in rural villages around Madrid (Spain) were processed to isolate rickettsiae. One sample containing mixtures of ticks and four containing eggs, in which rickettsiae had been detected by indirect immunofluorescence with a human serum highly reactive to Rickettsia conorii, were decontaminated, homogenized and inoculated onto Vero cells. Two egg samples yielded a cytopathic agent that reacted positively by immunofluorescence. One sample (14H) was successfully subcultured and identified as a member of the spotted fever group rickettsia. Tick eggs provide suitable material for isolation of rickettsia.

摘要

从马德里(西班牙)周边乡村地区的狗身上采集到的蜱虫被用于分离立克次体。一个含有蜱虫混合物的样本以及四个含有卵的样本(通过间接免疫荧光法,利用对康氏立克次体高度反应的人血清检测到其中存在立克次体)经过去污、匀浆处理后接种到Vero细胞上。两个含卵样本产生了一种通过免疫荧光呈阳性反应的细胞病变因子。一个样本(14H)成功传代培养,并被鉴定为斑点热群立克次体的一员。蜱虫卵为立克次体的分离提供了合适的材料。