大鼠聚（ADP - 核糖）聚合酶编码基因假定调控区的结构分析。

Structural analysis of the putative regulatory region of the rat gene encoding poly(ADP-ribose) polymerase.

作者信息

Potvin F, Thibodeau J, Kirkland J B, Dandenault B, Duchaine C, Poirier G G

机构信息

Laboratoire du métabolisme du poly(ADP-ribose), Endocrinologie moléculaire, Centre Hospitalier de l'Université Laval, Ste-Foy, Québec, Canada.

出版信息

FEBS Lett. 1992 May 18;302(3):269-73. doi: 10.1016/0014-5793(92)80457-r.

DOI:10.1016/0014-5793(92)80457-r

PMID:1601134

Abstract

A lambda EMBL3 clone containing the first three exons along with part of the 4th exon of the rat poly(ADP-ribose) polymerase gene was isolated from a genomic DNA library. This clone also contains 6.6 kbp of upstream sequences. Nucleotide sequence analysis of the proximal 5' 670 nucleotides flanking the major RNA start site of the rat gene does not reveal significant global homology with the same region of the human gene, but a series of short sequences are identical. Among these sequences are found two putative Sp1 binding sites along with a decanucleotide sequence responsible for the attachment of the transcription factor AP-2.

摘要

从基因组DNA文库中分离出一个λEMBL3克隆，它包含大鼠多聚（ADP - 核糖）聚合酶基因的前三个外显子以及第四个外显子的一部分。该克隆还包含6.6kbp的上游序列。对大鼠基因主要RNA起始位点侧翼的近端5' 670个核苷酸进行核苷酸序列分析，结果显示与人类基因的相同区域没有显著的整体同源性，但有一系列短序列是相同的。在这些序列中发现了两个假定的Sp1结合位点以及一个负责转录因子AP - 2附着的十核苷酸序列。