Sakkas D, Lacham O, Gianaroli L, Trounson A
Centre for Early Human Development, Monash University, Melbourne, Australia.
Fertil Steril. 1992 Jun;57(6):1279-88. doi: 10.1016/s0015-0282(16)55088-2.
To examine (1) fertilization rates obtained with subzonal sperm microinjection when different numbers of sperm are injected into the perivitelline space; (2) when subzonal sperm microinjection is combined with dilute insemination; and (3) the association of semen quality characteristics with fertilization.
Subzonal sperm microinjection and subzonal sperm microinjection combined with dilute insemination was performed in 109 and 41 cycles on patients in two clinical trials in Melbourne, Australia, and Bologna, Italy, respectively. PATIENT PARTICIPANTS: Couples who have experienced repeated in vitro fertilization failure or in whom the husband has severe male factor infertility.
The number of oocytes fertilized after injection of different numbers of sperm into the perivitelline space, the number of patients transferred, and pregnancy outcome.
The injection of multiple numbers of sperm into the perivitelline space failed to improve monospermic fertilization rates but caused an increase in polyspermic fertilization. In patients with initial semen parameters exhibiting greater than 50% motility or greater than 50% normal morphology fertilization rates were improved when subzonal sperm microinjection-treated eggs were incubated in a dilute insemination medium. Six pregnancies were obtained, two of which have progressed to term.
When applied to male factor patients, the subzonal sperm microinjection technique results in a 14% to 15% fertilization rate. However, of the 102 embryos transferred only three (2.9%) fetal heart beats were obtained.
研究（1）向卵周隙注射不同数量精子时，卵胞浆内单精子显微注射（ICSI）的受精率；（2）卵胞浆内单精子显微注射与稀释精液人工授精联合使用时的情况；（3）精液质量特征与受精的相关性。
在澳大利亚墨尔本和意大利博洛尼亚的两项临床试验中，分别对109例和41例患者进行了卵胞浆内单精子显微注射以及卵胞浆内单精子显微注射联合稀释精液人工授精。
经历过多次体外受精失败或丈夫患有严重男性因素不育症的夫妇。
向卵周隙注射不同数量精子后的受精卵母细胞数量、移植患者数量及妊娠结局。
向卵周隙注射多个精子未能提高单精子受精率，但会导致多精子受精增加。对于初始精液参数显示活力大于50%或正常形态大于50%的患者，当将卵胞浆内单精子显微注射处理的卵子在稀释精液人工授精培养基中孵育时，受精率有所提高。获得了6例妊娠，其中2例已足月。
应用于男性因素患者时，卵胞浆内单精子显微注射技术的受精率为14%至15%。然而，在移植的102个胚胎中，仅获得了3例（2.9%）有胎心搏动的情况。
