Zi Xiang-Dong, He Shi-Ming, Lu Hong, Feng Ji-An, Lu Jian-Yuan, Chang Shou, Wang Xiu
College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, PR China.
Anim Reprod Sci. 2006 Mar;92(1-2):183-92. doi: 10.1016/j.anireprosci.2005.05.017. Epub 2005 Jul 11.
The objectives of the present study were to evaluate the induction of estrus and fertility in yak cows treated with Co-Synch regimens or progesterone (P(4)). In Experiment 1, postpartum suckled yaks were assigned to three treatments: (1) A (n=28), insertion of an intravaginal device containing P(4) (CIDR) on Day 0, PGF(2alpha) (i.m.) on Day 6 and PMSG (i.m.) at the time of CIDR removal on Day 7 (P(4)-PGF(2alpha)-PMSG); (2) B (n=21), PGF(2alpha) (i.m.) on Day 6 and PMSG on Day 7; (3) C (n=26), control group. Seven yak bulls were grazed with the cows for natural breeding. Rate of estrus within 96h of the end of treatment was greater (P<0.05) in A (100.0%) than in B (28.6%) or C (0.0%). First service conception rate (CR) determined by serum P(4) on Day 21 after breeding was greater (P<0.05) in A (78.6%) than in B (22.2%). Also, pregnancy rate (PR) during the breeding season was greater (P<0.05) in A (82.1%) than in B (19.0%) and C (7.7%). In Experiment 2, non-suckled yaks that calved in previous years but not in the current year were assigned to three treatments: (1) A (n=31), GnRH (i.m.) on Day 0, followed by PGF(2alpha) on Day 7 and timed artificial insemination (TAI) concurrently with GnRH treatment on Day 9 (Co-Synch regimen); (2) B (n=50), a CIDR device for 7 days plus PGF(2alpha) and PMSG at the time of CIDR withdrawal on Day 7 and TAI on Day 9 (P(4)-PGF(2alpha)-PMSG); (3) C (n=50), yak cows were artificially inseminated at spontaneous estrus. Frozen semen of Holstein and Jersey were used for insemination in Experiment 2. The CR assessed by rectal palpation 35 days after TAI was not different in A (22.6%), B (30.0%) and C (33.3%), but PR was greater in A and B than in C, when based on those cows presented for estrous synchronization programs. It is concluded that P(4)-PGF(2alpha)-PMSG protocol could efficiently induce estrus and result in an acceptable pregnancy rate in postpartum suckled yak cows. This technique and Co-Synch regimen can be applied successfully for TAI of non-suckled yak cows.
本研究的目的是评估采用同期发情方案或孕酮（P4）处理的牦牛母牛的发情诱导和繁殖力。在实验1中，产后哺乳的牦牛被分为三个处理组：（1）A组（n = 28），在第0天插入含P4的阴道内装置（CIDR），第6天肌肉注射前列腺素F2α（PGF2α），第7天取出CIDR时肌肉注射孕马血清促性腺激素（PMSG）（P4 - PGF2α - PMSG）；（2）B组（n = 21），第6天肌肉注射PGF2α，第7天注射PMSG；（3）C组（n = 26），为对照组。7头牦牛公牛与母牛一起放牧进行自然交配。处理结束后96小时内的发情率，A组（100.0%）高于B组（28.6%）或C组（0.0%）（P < 0.05）。配种后第21天通过血清P4测定的首次配种受胎率，A组（78.6%）高于B组（22.2%）（P < 0.05）。此外，繁殖季节的妊娠率，A组（82.1%）高于B组（19.0%）和C组（7.7%）（P < 0.05）。在实验2中，前几年产犊但当年未产犊的非哺乳牦牛被分为三个处理组：（1）A组（n = 31），第0天肌肉注射促性腺激素释放激素（GnRH），第7天注射PGF2α，第9天与GnRH处理同时进行定时人工授精（TAI）（同期发情方案）；（2）B组（n = 50），放置CIDR装置7天，第7天取出CIDR时注射PGF2α和PMSG，第9天进行TAI（P4 - PGF2α - PMSG）；（3）C组（n = 50），牦牛母牛在自然发情时进行人工授精。实验2中使用荷斯坦和泽西牛的冷冻精液进行授精。TAI后35天通过直肠触诊评估的受胎率，A组（22.6%）、B组（30.0%）和C组（33.3%）之间无差异，但基于参与发情同步程序的母牛，A组和B组的妊娠率高于C组。结论是，P4 - PGF2α - PMSG方案可有效诱导产后哺乳牦牛母牛发情并产生可接受的妊娠率。该技术和同期发情方案可成功应用于非哺乳牦牛母牛的TAI。
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