Chlebicki Maciej Piotr, Tan Ban Hock, Koh Tse Hsien
Department of Infectious Diseases, Singapore General Hospital, Singapore.
Scand J Infect Dis. 2005;37(6-7):517-20. doi: 10.1080/00365540410021090.
We present the case of a 51-y-old male with multiple splenic abscesses that failed to resolve despite antimicrobial therapy targeted at Burkholderia pseudomallei. Elective splenectomy was performed to clarify the diagnosis. Pus from the spleen was subjected to universal bacterial 16S rDNA amplification and sequencing. The sequence obtained was 99% identical to 16S rDNA sequences for Burkholderia pseudomallei. Sequencing of the 16S rDNA is a very promising diagnostic tool allowing quick identification of Burkholderia pseudomallei directly from clinical specimen.
我们报告了一例51岁男性患者，其患有多个脾脓肿，尽管针对类鼻疽伯克霍尔德菌进行了抗菌治疗，但脓肿仍未消退。为明确诊断，进行了择期脾切除术。从脾脏获取的脓液进行了通用细菌16S rDNA扩增和测序。所得序列与类鼻疽伯克霍尔德菌的16S rDNA序列有99%的同一性。16S rDNA测序是一种非常有前景的诊断工具，可直接从临床标本中快速鉴定类鼻疽伯克霍尔德菌。
