Ko Woo Shin, Park Tae Yeol, Park Cheol, Kim Young Hee, Yoon Hwa Jung, Lee Seung Yeon, Hong Sang Hoon, Choi Byung Tae, Lee Yong Tae, Choi Yung Hyun
Department of Oriental Medicine, Dongeui University College of Oriental Medicine and Clinical Research Center of Oriental Medicine, Busan 614-052, Korea.
Oncol Rep. 2005 Aug;14(2):475-80.
Chan Su is a traditional Chinese medicine prepared from the dried white secretion of the auricular and skin glands of toads, and has been used as an Oriental drug. However, little is known about the effect of Chan Su on the growth of human cancer cells. This study was undertaken to investigate the underlying mechanism of Chan Su-induced apoptosis in a human bladder carcinoma cell line, T24. The effects of this compound were also tested on cyclooxygenase (COX) activity. Treatment of T24 cells with Chan Su resulted in the inhibition of viability and induction of apoptosis in a concentration-dependent manner, which was proved by trypan blue counts, DAPI staining, agarose gel electrophoresis and flow cytometric analysis. Apoptosis of T24 cells by Chan Su was associated with a down-regulation of anti-apoptotic Bcl-2 and Bcl-X(S/L) expression and an up-regulation of pro-apoptotic Bax expression. Chan Su treatment induced the proteolytic activation of caspase-3 and caspase-9, and a concomitant degradation of poly(ADP-ribose)-polymerase and beta-catenin protein. Furthermore, Chan Su decreased the levels of COX-2 mRNA and protein expression without significant changes in the levels of COX-1, which was correlated with an inhibition in prostaglandin E(2) synthesis. Taken together, these findings partially provide novel insights into the possible molecular mechanisms of the anti-cancer activity of Chan Su.
蟾酥是一种由蟾蜍耳后腺及皮肤腺的干燥白色分泌物制成的传统中药，一直被用作一种东方药物。然而，关于蟾酥对人癌细胞生长的影响知之甚少。本研究旨在探讨蟾酥诱导人膀胱癌细胞系T24凋亡的潜在机制。还检测了该化合物对环氧合酶（COX）活性的影响。用蟾酥处理T24细胞导致细胞活力受到抑制并以浓度依赖性方式诱导凋亡，这通过台盼蓝计数、DAPI染色、琼脂糖凝胶电泳和流式细胞术分析得到证实。蟾酥诱导T24细胞凋亡与抗凋亡蛋白Bcl-2和Bcl-X（S/L）表达下调以及促凋亡蛋白Bax表达上调有关。蟾酥处理诱导了半胱天冬酶-3和半胱天冬酶-9的蛋白水解激活，以及聚（ADP-核糖）-聚合酶和β-连环蛋白的伴随降解。此外，蟾酥降低了COX-2 mRNA和蛋白表达水平，而COX-1水平没有显著变化，这与前列腺素E2合成受到抑制相关。综上所述，这些发现部分地为蟾酥抗癌活性的可能分子机制提供了新的见解。
