Weikert Steffen, Christoph Frank, Köllermann Jens, Müller Markus, Schrader Mark, Miller Kurt, Krause Hans
Charitè - University Medicine in Berlin, Campus Benjamin Franklin, Department of Urology, Hindenburgdamm 30, D-12200 Berlin, Germany.
Int J Mol Med. 2005 Aug;16(2):349-53.
The polycomb group protein enhancer of zeste 2 (EZH2) is a transcriptional repressor involved in the control of cellular proliferation and oncogenesis. The aim of the present study was to quantify EZH2 expression in bladder carcinomas and to correlate the data with clinicopathological findings. EZH2 mRNA expression was measured by real-time reverse transcription-polymerase chain reaction in tumor tissue specimens obtained from 37 patients with urothelial carcinomas of the bladder and in four bladder cancer cell lines. EZH2 levels were normalized to expression of the housekeeping porphobilinogen deaminase gene. EZH2 transcripts were commonly detected in tumor tissue. Transcript levels correlated significantly with the invasiveness of bladder tumors (p = 0.029) with elevated EZH2 mRNA expression measured in invasive bladder carcinomas (median value, 38.92) compared with non-invasive tumors (15.51). In addition, levels of expression were significantly higher in high-grade (G3) than in low-grade (G1/2) lesions (p < 0.001). EZH2 mRNA levels in bladder carcinoma cell lines were within the range of high-grade invasive bladder cancers. In conclusion, expression levels of EZH2 are elevated in aggressive and invasive urothelial carcinomas, suggesting that deregulated EZH2 expression may be involved in the progression of bladder tumors.
多梳蛋白家族成员zeste 2增强子（EZH2）是一种转录抑制因子，参与细胞增殖和肿瘤发生的调控。本研究旨在定量检测EZH2在膀胱癌中的表达，并将数据与临床病理结果相关联。采用实时逆转录聚合酶链反应检测了37例膀胱尿路上皮癌患者肿瘤组织标本及4种膀胱癌细胞系中EZH2 mRNA的表达。EZH2水平以管家基因胆色素原脱氨酶的表达进行标准化。肿瘤组织中普遍检测到EZH2转录本。转录水平与膀胱肿瘤的侵袭性显著相关（p = 0.029），侵袭性膀胱癌中EZH2 mRNA表达升高（中位数为38.92），而无侵袭性肿瘤中为（15.51）。此外，高级别（G3）病变中的表达水平显著高于低级别（G1/2）病变（p < 0.001）。膀胱癌细胞系中EZH2 mRNA水平处于高级别侵袭性膀胱癌范围内。总之，EZH2在侵袭性膀胱尿路上皮癌中的表达水平升高，提示EZH2表达失调可能参与膀胱肿瘤的进展。
