Adams Yvonne, Smith Simone L, Schwartz-Albiez Reinhard, Andrews Katherine T
Hygiene-Institut, Abteilung Parasitologie, Universität Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany.
Parasitol Res. 2005 Oct;97(4):290-4. doi: 10.1007/s00436-005-1426-3. Epub 2005 Jul 13.
Carbohydrates are implicated in many of the invasive and adhesive interactions that occur between Plasmodium falciparum malaria parasites and human host cells, including invasion of sporozoites into hepatocytes, entry of merozoites into new host erythrocytes during asexual blood-stage replication, adhesion of infected erythrocytes to uninfected erythrocytes (rosetting) and to a number of host endothelial receptors including ICAM-1, CD36 and chondroitin-4-sulphate. In addition to increasing our understanding of host-parasite interactions, the investigation of carbohydrates with differing levels and patterns of sulphation as inhibitors may contribute to the development of novel therapeutics targeting malaria. Here we show that three polysaccharides derived from seaweed (carrageenans) with differing sulphation levels and patterns can inhibit the in vitro erythrocytic invasion and growth of both drug sensitive and drug resistant P. falciparum lines and the adhesion of parasitized erythrocytes to the human glycoprotein CD36.
碳水化合物与恶性疟原虫疟原虫和人类宿主细胞之间发生的许多侵袭性和粘附性相互作用有关，包括子孢子侵入肝细胞、裂殖子在无性血液阶段复制期间进入新的宿主红细胞、受感染红细胞与未感染红细胞的粘附（形成玫瑰花结）以及与包括细胞间粘附分子-1（ICAM-1）、CD36和硫酸软骨素-4-硫酸盐在内的多种宿主内皮受体的粘附。除了增进我们对宿主-寄生虫相互作用的理解外，研究具有不同硫酸化水平和模式的碳水化合物作为抑制剂可能有助于开发针对疟疾的新型疗法。在这里，我们表明，三种来自海藻的多糖（角叉菜胶）具有不同的硫酸化水平和模式，可以抑制药物敏感和耐药恶性疟原虫株的体外红细胞侵袭和生长，以及被寄生红细胞与人类糖蛋白CD36的粘附。
