粗糙脉孢菌谷氨酰胺合成酶。在源自兔网织红细胞的无细胞系统中特定信使核糖核酸的翻译。

Neurospora crassa glutamine synthetase. Translation of specific messenger ribonucleic acid in a cell-free system derived from rabbit reticulocytes.

作者信息

Palacios R, Campomanes M, Quinto C

出版信息

J Biol Chem. 1977 May 10;252(9):3028-34.

Abstract

The total reticulocyte lysate cell-free protein-synthesizing system was incubated in the presence of Neurospora crassa RNA. With the aid of an antibody directed against purified N. crassa glutamine synthetase, the synthesis of a specific protein was detected. This protein precipitates with antiglutamine synthetase using both direct and indirect procedures, migrates with the same molecular weight as the monomer of N. crassa glutamine synthetase when subjected to acrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, and chromatographs as N. crassa glutamine synthetase on anthranilate-bound Sepharose. These data indicate the translation of the mRNA that codes for N. crassa glutamine synthetase. This RNA behaves as poly(A)-containing material when fractionated on oly(U)-Sepha-rose.

摘要

将总的网织红细胞裂解物无细胞蛋白质合成系统在粗糙脉孢菌RNA存在的情况下进行温育。借助针对纯化的粗糙脉孢菌谷氨酰胺合成酶的抗体，检测到一种特异性蛋白质的合成。使用直接和间接方法，这种蛋白质都能与抗谷氨酰胺合成酶一起沉淀，在十二烷基硫酸钠存在下进行丙烯酰胺凝胶电泳时，其迁移的分子量与粗糙脉孢菌谷氨酰胺合成酶单体相同，并且在邻氨基苯甲酸结合的琼脂糖上进行色谱分析时表现为粗糙脉孢菌谷氨酰胺合成酶。这些数据表明了编码粗糙脉孢菌谷氨酰胺合成酶的mRNA的翻译。当在寡聚（U）-琼脂糖上进行分级分离时，这种RNA表现为含聚（A）的物质。