Liu Hui-jun, Zhan Xiu-Ming, Li Ke-bin, Liu Wei-ping
Institute of Environmental Science, Zhejiang University, Hangzhou 310027, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Mar;25(3):463-6.
The relationship between excess thermodynamic function and binding to urease of four chloroacetanilide herbicides was studied using high-performance liquid chromatography and fluorescence spectrum. The linear relationship between the composition of mobile phase of RP-HPLC and the capacity factor of chloroacetanilide herbicides has been obtained. The excess thermodynamic enthalpy (deltaH* ) of acetochlor, pretilachlor, butachlor and metolachlor was determined, and the binding constant K and the number of binding sites with urease were calculated. The relationship between excess thermodynamic function and the binding constant K was suggested.
