Kessler Harald H
Molecular Diagnostics Laboratory and National Reference Laboratory for Hepatitis A, B, C, Institute of Hygiene, Medical University Graz, Universitaetsplatz 4, A-8010 Graz, Austria.
Expert Rev Mol Diagn. 2005 Jul;5(4):531-6. doi: 10.1586/14737159.5.4.531.
Infection with the hepatitis B virus (HBV) continues to present diagnostic and therapeutic challenges worldwide. Today, many routine diagnostic laboratories have implemented assays based on molecular techniques for the detection of HBV DNA. However, the standard algorithm for specific diagnosis of HBV infection still relies on serologic testing. Molecular assays are employed for pretreatment evaluation, clinical staging and monitoring of antiviral therapy. Furthermore, molecular methods are essential for identification of mutations in the HBV genome. Although a continuous improvement of assay performance has been observed during recent years, lack of comparability of different molecular assays remains a problem to be resolved in the future. The limited range of linearity when employing conventional PCR will be overcome by using real-time assays.
乙肝病毒（HBV）感染在全球范围内仍然带来诊断和治疗方面的挑战。如今，许多常规诊断实验室已采用基于分子技术的检测方法来检测HBV DNA。然而，HBV感染的特异性诊断标准算法仍依赖血清学检测。分子检测用于抗病毒治疗的预处理评估、临床分期和监测。此外，分子方法对于鉴定HBV基因组中的突变至关重要。尽管近年来检测性能不断提高，但不同分子检测方法缺乏可比性仍是未来有待解决的问题。采用实时检测将克服传统PCR线性范围有限的问题。
