柯萨奇病毒和腺病毒受体（CAR）在正常及再生人肌肉中的定位

Localization of coxsackie virus and adenovirus receptor (CAR) in normal and regenerating human muscle.

作者信息

Sinnreich M, Shaw C A, Pari G, Nalbantoglu J, Holland P C, Karpati G

机构信息

Neuromuscular Research Group, Montreal Neurological Hospital and Institute, McGill University, 3801 University Street, Montreal, Que., Canada H3A 2B4.

出版信息

Neuromuscul Disord. 2005 Aug;15(8):541-8. doi: 10.1016/j.nmd.2005.05.007.

DOI:10.1016/j.nmd.2005.05.007

PMID:16014330

Abstract

The primary receptor for Adenovirus and Coxsackie virus (CAR) serves as main port of entry of the adenovirus vector mediating gene transfer into skeletal muscle. Information about CAR expression in normal and diseased human skeletal muscle is lacking. C'- or N'-terminally directed polyclonal antibodies against CAR were generated and immunohistochemical analysis of CAR on morphologically normal and regenerating human skeletal muscle of children and adults was performed. In morphologically normal human muscle fibers, CAR immunoreactivity was limited to the neuromuscular junction. In regenerating muscle fibers, CAR was abundantly co-expressed with markers of regeneration. The function of CAR at the neuromuscular junction is currently unknown. Co-expression of CAR with markers of regeneration suggests that CAR is developmentally regulated, and may serve as a marker of skeletal muscle fiber regeneration.

摘要

腺病毒和柯萨奇病毒的主要受体（CAR）是介导基因转移的腺病毒载体进入骨骼肌的主要入口。目前尚缺乏关于CAR在正常和患病人类骨骼肌中表达的信息。我们制备了针对CAR的C端或N端定向多克隆抗体，并对儿童和成人形态学正常及再生的人类骨骼肌进行了CAR的免疫组织化学分析。在形态学正常的人类肌纤维中，CAR免疫反应仅限于神经肌肉接头。在再生肌纤维中，CAR与再生标志物大量共表达。目前尚不清楚CAR在神经肌肉接头处的功能。CAR与再生标志物的共表达表明CAR受发育调控，可能作为骨骼肌纤维再生的标志物。