Mita Giovanni, Quarta Angela, Fasano Pasqua, De Paolis Angelo, Di Sansebastiano Gian Pietro, Perrotta Carla, Iannacone Rina, Belfield Eric, Hughes Richard, Tsesmetzis Nicolas, Casey Rod, Santino Angelo
Institute of Sciences of Food Production CNR Section of Lecce, via Monteroni, I-73100 Italy.
J Exp Bot. 2005 Sep;56(419):2321-33. doi: 10.1093/jxb/eri225. Epub 2005 Jul 12.
Oxylipin metabolism represents one of many defence mechanisms employed by plants. It begins with the oxygenation of polyunsaturated fatty acids by lipoxygenases to form fatty acid hydroperoxides that are substrates for several enzymes, including specialized cytochrome P450s known as CYP74s. The targeting of a new CYP74, a 9-hydroperoxide lyase (HPL) from almonds, to the endomembrane system and lipid bodies, both as enzyme activity in almond seeds and as GFP fusions transiently expressed in tobacco protoplasts, is described. Such association of a CYP74 with lipid bodies has not been reported previously. Also described are the properties of a 9-HPL gene, the developmental regulation of its expression, the production and characterization of recombinant 9-HPL in Escherichia coli, and the developmental correlation between gene expression, enzyme activity, and the appearance of volatile C9 aldehydes from HPL action.
氧脂代谢是植物采用的众多防御机制之一。它始于脂氧合酶对多不饱和脂肪酸的氧化作用，形成脂肪酸氢过氧化物，这些氢过氧化物是几种酶的底物，包括被称为CYP74s的特殊细胞色素P450。本文描述了一种来自杏仁的新型CYP74，即9-氢过氧化物裂解酶（HPL），在杏仁种子中作为酶活性以及在烟草原生质体中瞬时表达的绿色荧光蛋白融合体，定位于内膜系统和脂质体的情况。此前尚未报道过CYP74与脂质体的这种关联。本文还描述了9-HPL基因的特性、其表达的发育调控、在大肠杆菌中重组9-HPL的生产和表征，以及基因表达、酶活性与HPL作用产生的挥发性C9醛外观之间的发育相关性。
