Nordhoff Marcel, Taras David, Macha Moritz, Tedin Karsten, Busse Hans-Jürgen, Wieler Lothar H
Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, D-10115 Berlin, Germany.
Institut für Tierernährung, Freie Universität Berlin, D-10115 Berlin, Germany.
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1675-1680. doi: 10.1099/ijs.0.63388-0.
Limit-dilution procedures were used to isolate seven, helically coiled bacterial strains from faeces of swine that constituted two unidentified taxa. Comparative 16S rRNA gene sequence analysis showed highest similarity values with species of the genus Treponema indicating that the isolates are members of this genus. Strain 7CPL208(T), as well as five further isolates, and 14V28(T) displayed the highest 16S rRNA gene sequence similarities with Treponema pectinovorum ATCC 33768(T) (92.3%) and Treponema parvum OMZ 833(T) (89.9%), respectively. Polar lipid profiles distinguished 7CPL208(T) and 14V28(T) from each other as well as from related species. Based on their phenotypic and genotypic distinctiveness, strains 7CPL208(T) and 14V28(T) are suggested to represent two novel species of the genus Treponema, for which the names Treponema berlinense sp. nov. and Treponema porcinum sp. nov. are proposed. The type strain for Treponema berlinense is 7CPL208(T) (=ATCC BAA-909(T)=CIP 108244(T)=JCM 12341(T)) and for Treponema porcinum 14V28(T) (=ATCC BAA-908(T)=CIP 108245(T)=JCM 12342(T)).
采用有限稀释法从猪粪便中分离出7株螺旋状细菌菌株，它们分属于两个未鉴定的分类单元。16S rRNA基因序列比较分析表明，这些分离菌株与密螺旋体属物种的相似性最高，表明它们是该属的成员。菌株7CPL208(T)以及另外5株分离菌株，分别与果胶密螺旋体ATCC 33768(T)（92.3%）和细小密螺旋体OMZ 833(T)（89.9%）显示出最高的16S rRNA基因序列相似性。极性脂质谱将7CPL208(T)和14V28(T)彼此区分开来，也与相关物种区分开来。基于它们在表型和基因型上的独特性，建议菌株7CPL208(T)和14V28(T)代表密螺旋体属的两个新物种，分别命名为柏林密螺旋体（Treponema berlinense sp. nov.）和猪密螺旋体（Treponema porcinum sp. nov.）。柏林密螺旋体的模式菌株为7CPL208(T)（=ATCC BAA-909(T)=CIP 108244(T)=JCM 12341(T)），猪密螺旋体的模式菌株为14V28(T)（=ATCC BAA-908(T)=CIP 108245(T)=JCM 12342(T)）。
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