Smith Jodie, Onley David, Garey Caroline, Crowther Stuart, Cahir Nicholas, Johanson Adam, Painter Sianie, Harradence Grant, Davis Ricardo, Swarbrick Peter
SmartBead Technologies Limited, Babraham Hall, Babraham, Cambridge CB2 4AT, United Kingdom.
Ann N Y Acad Sci. 2005 Jun;1050:286-94. doi: 10.1196/annals.1313.030.
SmartBead Technologies has developed a multiplexed immunofluorescence assay, the UltraPlex ANA Profile, which determines nine antinuclear antibodies simultaneously. The UltraPlex assay platform uses bar-coded microparticles to track analytes through assays. These bar-coded microparticles were used to tag and track key ANA markers: Jo-1, Scl-70, Sm, SmRNP, SSA, SSB, U1RNP, Centromere B, dsDNA, and a blank control microparticle. The immunofluorescence assays are fully automated and are performed on a Perkin-Elmer multiprobe II liquid handling system that performs all sera dilutions, additions of reagents, washes, and incubation steps. Results were determined by the automated UltraPlex plate reader. This fully automated multiplex antinuclear antibody (ANA) immunoassay was used to screen commercially available ANA-positive sera and negative control samples. The UltraPlex ANA Profile enables the panels of samples to be screened simultaneously for nine ANA antoantibodies, requiring significantly less labor and fewer reagents, with performance equivalent to existing gold-standard methods.
SmartBead Technologies公司开发了一种多重免疫荧光检测方法——UltraPlex ANA Profile，可同时检测九种抗核抗体。UltraPlex检测平台使用条形码微珠在检测过程中追踪分析物。这些条形码微珠用于标记和追踪关键的抗核抗体标志物：Jo-1、Scl-70、Sm、SmRNP、SSA、SSB、U1RNP、着丝粒B、双链DNA，以及一个空白对照微珠。免疫荧光检测完全自动化，在珀金埃尔默多探针II液体处理系统上进行，该系统可完成所有血清稀释、试剂添加、洗涤和孵育步骤。结果由自动化的UltraPlex酶标仪测定。这种全自动多重抗核抗体（ANA）免疫检测方法用于筛查市售的ANA阳性血清和阴性对照样本。UltraPlex ANA Profile能够同时对样本进行九种抗核抗体自身抗体的筛查，所需劳动力显著减少，试剂用量更少，性能与现有的金标准方法相当。
