Bencimon Celine, Salles Gilles, Moreira Annick, Guyomard Stephanie, Coiffier Bertrand, Bienvenu Jacques, Fabien Nicole
Laboratoire d'Auto-Immunité, Centre Hospitalier Lyon-Sud (Hospices Civils de Lyon), Chemin du Grand Revoyet, 69495 Pierre-Bénite cedex, France.
Ann N Y Acad Sci. 2005 Jun;1050:319-26. doi: 10.1196/annals.1313.034.
An association between autoimmunity and hematological malignancies has been reported including the detection of antinuclear autoantibodies (ANAs) in patients suffering from non-Hodgkin's lymphoma (NHL), with a high prevalence of ANAs directed to components of the mitotic apparatus or the mitosis-associated proteins. Previous studies have demonstrated that one of the targets of such ANAs could be the CENP-F protein, especially in some carcinomas. The prevalence and specificity of anti-CENP-F autoantibodies (aAbs) thus were analyzed in 347 patients with different histological subgroups of NHL before any treatment of NHL, along with 150 controls. The detection of these aAbs was performed using two techniques: a radioimmunological assay (RIA) and an indirect immunofluorescence technique (IIF). Twenty-five (7.2%) NHL patients and 2 (1.3%) control patients displayed anti-CENP-F aAbs using RIA. This difference between the two groups was found to be significant (P < 0.01), with a higher prevalence of aAbs in the follicular (13%) and in the marginal zone B and MALT (10.2%) lymphoma subgroups. By IIF, 10 (2.9%) patients with NHL displayed aAbs with a CENP-F or CENP-F-like pattern, whereas none of the control group did. In conclusion, these data demonstrate that a significant incidence of anti-CENP-F aAbs is observed, before any treatment, in some histological subgroups of NHL patients. In addition to the usefulness of anti-CENP-F aAbs as a marker for some NHL subgroups, prospective studies may be important to evaluate the predictive value of anti-CENP-F aAbs for the development of carcinomas.
已有报道称自身免疫与血液系统恶性肿瘤之间存在关联，包括在非霍奇金淋巴瘤（NHL）患者中检测到抗核自身抗体（ANA），其中针对有丝分裂装置成分或有丝分裂相关蛋白的ANA患病率较高。先前的研究表明，此类ANA的靶标之一可能是CENP-F蛋白，尤其是在某些癌症中。因此，在347例未经任何NHL治疗的不同组织学亚组的NHL患者以及150例对照中，分析了抗CENP-F自身抗体（aAb）的患病率和特异性。使用两种技术检测这些aAb：放射免疫测定（RIA）和间接免疫荧光技术（IIF）。使用RIA时，25例（7.2%）NHL患者和2例（1.3%）对照患者显示出抗CENP-F aAb。发现两组之间的这种差异具有统计学意义（P < 0.01），在滤泡性淋巴瘤（13%）以及边缘区B细胞淋巴瘤和黏膜相关淋巴组织淋巴瘤（MALT，10.2%）亚组中aAb的患病率更高。通过IIF检测，10例（2.9%）NHL患者显示出具有CENP-F或CENP-F样模式的aAb，而对照组中无一例出现。总之，这些数据表明，在未经任何治疗的情况下，在某些组织学亚组的NHL患者中观察到抗CENP-F aAb的发生率较高。除了抗CENP-F aAb作为某些NHL亚组标志物的有用性之外，前瞻性研究对于评估抗CENP-F aAb对癌症发生的预测价值可能很重要。
