苜蓿银纹夜蛾核型多角体病毒的立即早期蛋白IE0对病毒复制并非必需。
The immediate-early protein IE0 of the Autographa californica nucleopolyhedrovirus is not essential for viral replication.
作者信息
Lu Liqun, Rivkin Hadassah, Chejanovsky Nor
机构信息
Entomology Department, The Institute of Plant Protection, The Volcani Center, Bet Dagan, Israel.
出版信息
J Virol. 2005 Aug;79(15):10077-82. doi: 10.1128/JVI.79.15.10077-10082.2005.
Abstract
The role of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein IE0 in the baculoviral infection is not clear. In this study, we constructed the recombinant virus vAcDeltaie0 null for ie0 expression by targeted mutagenesis replacing exon0 with the cat gene. We found that vAcDeltaie0 replicated efficiently in Spodoptera littoralis SL2 cells, which are poorly permissive for AcMNPV. In contrast, in Spodoptera frugiperda SF9 cells, which are fully permissive for AcMNPV, vAcDeltaie0 DNA replication and budded virus production were delayed. These results and recently published data (X. Dai et al., J. Virol. 78:9633-9644, 2004) indicate that ie0 is not essential for AcMNPV replication but enhances it in permissive SF9 cells.
摘要
苜蓿银纹夜蛾多粒包埋核型多角体病毒（AcMNPV）的立即早期蛋白IE0在杆状病毒感染中的作用尚不清楚。在本研究中，我们通过用cat基因替换外显子0进行靶向诱变，构建了用于ie0表达的重组病毒vAcDeltaie0缺失株。我们发现vAcDeltaie0在对AcMNPV感染性较差的斜纹夜蛾SL2细胞中能高效复制。相比之下，在对AcMNPV完全敏感的草地贪夜蛾SF9细胞中，vAcDeltaie0的DNA复制和出芽病毒产生均延迟。这些结果以及最近发表的数据（X. Dai等人，《病毒学杂志》78:9633 - 9644，2004年）表明，ie0对于AcMNPV复制并非必需，但在敏感的SF9细胞中能增强其复制。
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