Chejanovsky N, Gershburg E
Entomology Department, Volcani Center, Dagan, Israel.
Virology. 1995 Jun 1;209(2):519-25. doi: 10.1006/viro.1995.1284.
Spodoptera littoralis cells infected with the Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) yielded significantly lower budded virus titers than Spodoptera frugiperda-infected cells and produced very low levels of polyhedrin. Relative to AcMNPV-infected S. frugiperda SF9 cells viral DNA replication was severely reduced in Spodoptera littoralis SL2 cells. Microscopic examination of SL2-infected cells revealed progressive cell blebbing starting at 6-8 hr postinfection and culminating in total cell destruction at 24 hr postinfection. The data suggested that AcMNPV-infected SL2 cells undergo apoptosis. The occurrence of an active apoptotic process in the infected cells was confirmed by: (1) observation of fragmentation of the cell nuclei stained with the specific fluorescent dye DAPI (4',6'-diamidino-2-phenylindole) and (2) the presence of low-molecular-weight DNA oligomers. Neither SL2 cells infected with S. littoralis nuclear polyhedrosis virus (SINPV) nor SF9 cells infected with AcMNPV, respectively, showed nuclear fragmentation or oligonucleosomal ladder formation.
感染了苜蓿银纹夜蛾多核多角体病毒（AcMNPV）的草地贪夜蛾细胞产生的出芽病毒滴度明显低于感染该病毒的草地贪夜蛾细胞，且多角体蛋白水平极低。相对于感染AcMNPV的草地贪夜蛾SF9细胞，草地贪夜蛾SL2细胞中的病毒DNA复制严重减少。对感染SL2细胞的显微镜检查显示，感染后6 - 8小时开始出现渐进性细胞起泡，并在感染后24小时导致细胞完全破坏。数据表明，感染AcMNPV的SL2细胞会发生凋亡。感染细胞中活跃凋亡过程的发生通过以下方式得到证实：（1）观察用特异性荧光染料4',6'-二脒基-2-苯基吲哚（DAPI）染色的细胞核的碎片化，以及（2）低分子量DNA寡聚体的存在。分别感染草地贪夜蛾核型多角体病毒（SINPV）的SL2细胞和感染AcMNPV的SF9细胞均未显示细胞核碎片化或寡核小体梯带形成。
