[利用发根农杆菌生产转基因甜菜植株（Beta vulgaris L.）]
[Production of transgenic sugarbeet plants (Beta vulgaris L.) using Agrobacterium rhizogenes].
作者信息
Kishchenko E M, Komarnitskiĭ I K, Kuchuk N V
出版信息
Tsitol Genet. 2005 Jan-Feb;39(1):9-13.
PMID:16018172
Abstract
Normal phenotype sugarbeet plants transformed with Agrobacterium rhizogenes were produced using direct regeneration from explants without hairy root phase. Kanamycin resistant plants and Ri-roots carrying the genes of neomycin phosphotransferase II and b-glucuronidase have been obtained. Integration of transgenes into sugarbeet genome was confirmed with GUS-assay and PCR using primers for the introduced genes.
摘要
利用外植体直接再生法，在不经过毛状根阶段的情况下，培育出了用发根农杆菌转化的具有正常表型的甜菜植株。已获得了携带新霉素磷酸转移酶II基因和β-葡萄糖醛酸酶基因的卡那霉素抗性植株和发根。通过GUS检测和使用导入基因引物的PCR，证实了转基因已整合到甜菜基因组中。
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