Shi He-Ping, Liang Peng, Quan Hong
College of Life Science, South China Normal University, Guangzhou 510631, China.
Sheng Wu Gong Cheng Xue Bao. 2003 Jan;19(1):46-9.
Hairy roots appeared in vitro 20 days after inoculation of Phytolacca esculenta leaf explants with the strain of Agrobacterium rhizogenes R1601. The frequency of leaf explants transformed by R1601 was up to 70%. Hairy roots can be incited directly from the veins of explants or via callus. Hairy roots induced by R1601 grew rapidly on medium MS without hormone and were 85.6% higher in respiration rate than control roots. Transformation was confirmed by opine detection and the amplification of rol B and rol C genes from the hairy roots of P. esculenta. The total saponin content in hairy roots of P. esculenta was about 1.54 times as much as in natural roots whereas polysaccharides content was about 70% times as much as in the natural roots.
用发根农杆菌R1601菌株接种商陆叶片外植体20天后，体外出现了毛状根。R1601转化叶片外植体的频率高达70%。毛状根可直接从外植体的叶脉诱导产生，也可通过愈伤组织诱导产生。R1601诱导的毛状根在不含激素的MS培养基上生长迅速，呼吸速率比对照根高85.6%。通过冠瘿碱检测以及从商陆毛状根中扩增rol B和rol C基因来确认转化。商陆毛状根中的总皂苷含量约为天然根的1.54倍，而多糖含量约为天然根的70%。
