Shi Fang, Liu Kun-Fan, Endo Takashi R, Wang Dao-Wen
The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Yi Chuan Xue Bao. 2005 May;32(5):487-94.
To generate 1 R deletion and translocation lines, we introduced a 2C chromosome,which was derived from Aegilops cylindrica and was known to have a gametocidal function when added monosomically into common wheat cv. Chinese Spring (CS) and its derivative, into a wheat-rye 1R chromosome disomic addition line (CS-1R"). When the individuals with chromosome constitution 21" + 1R" + 2C' (2n = 45) were selfed, the 1R chromosome structural changes were found to be induced with high frequency (24.1%) among the progenies. By using C-banding and GISH analysis, we analyzed 1R structural changes in 46 F3 individuals, which came from 23 F2 plants. The rearranged 1R chromosomes could be characterized in about 85% of the F3 individuals. This included telosome 1RL (39.1%), iso-chromosome 1 RL (2.2%), whole arm translocation involving 1RL (32.6%), telosome 1RS (4.3%), iso-chromosome 1RS (4.3%), and 1R deletion mutant with break point in the long arm (2.2%). The mutant 1R lines obtained in this study will potentially be useful in mapping the chromosome locations of agronomically important genes located in 1R. This study also demonstrated that molecular markers might be used to identify wheat chromosome arm involved in translocation with 1R.
为了创建1R染色体缺失和易位系，我们导入了一条2C染色体，该染色体源自圆柱山羊草，已知其单倍体添加到普通小麦品种中国春（CS）及其衍生品种中时具有杀配子功能。我们将其导入一个小麦-黑麦1R染色体二体附加系（CS-1R”）中。当染色体组成为21” + 1R” + 2C’（2n = 45）的个体自交时，在其后代中发现1R染色体结构变化的诱导频率很高（24.1%）。通过C带和基因组原位杂交（GISH）分析，我们对来自23株F2植株的46个F3个体的1R结构变化进行了分析。在约85%的F3个体中可以鉴定出重排的1R染色体。这包括1RL端体（39.1%）、1RL等臂染色体（2.2%）、涉及1RL的整条染色体臂易位（32.6%）、1RS端体（4.3%）、1RS等臂染色体（4.3%）以及长臂上有断点的1R缺失突变体（2.2%）。本研究中获得的突变1R系可能有助于定位位于1R染色体上的重要农艺基因的染色体位置。本研究还表明，分子标记可用于鉴定与1R发生易位的小麦染色体臂。
