使用嵌合感染性克隆鉴定影响西尼罗河（萨拉芬德）病毒顺式成熟模式的区域。

Identifying the region influencing the cis-mode of maturation of West Nile (Sarafend) virus using chimeric infectious clones.

作者信息

Li J, Bhuvanakantham R, Howe J, Ng M-L

机构信息

Flavivirology Laboratory, Department of Microbiology, National University of Singapore, Singapore 117597, Singapore.

出版信息

Biochem Biophys Res Commun. 2005 Aug 26;334(2):714-20. doi: 10.1016/j.bbrc.2005.06.150.

DOI:10.1016/j.bbrc.2005.06.150

PMID:16018972

Abstract

West Nile (Sarafend) virus [WN(S)V] has been shown to egress by budding at the plasma membrane of infected cells. However, the region influencing this mode of virus release remains to be deciphered. In this study, we have constructed three chimeric clones in which specific regions of West Nile (Wengler) virus [WN(W)V] were replaced for the corresponding regions of WN(S)V in the full-length infectious clone of WN(S)V to define the region responsible for the cis-mode of WN(S)V maturation. The WN(W)V matures by the trans-mode. All of the resulting chimeric viruses were found to be infective. Transmission electron microscopy analyses performed in Vero cells infected with these chimeric viruses disclosed that the 5' end of the WN(S)V genome plays a major role in influencing the process of maturation at the plasma membrane.

摘要

西尼罗河（萨拉芬德）病毒[WN(S)V]已被证明通过在受感染细胞的质膜上出芽而释放。然而，影响这种病毒释放模式的区域仍有待破解。在本研究中，我们构建了三个嵌合克隆，其中西尼罗河（温格勒）病毒[WN(W)V]的特定区域被替换为WN(S)V全长感染性克隆中WN(S)V的相应区域，以确定负责WN(S)V成熟顺式模式的区域。WN(W)V通过反式模式成熟。所有产生的嵌合病毒都具有感染性。在用这些嵌合病毒感染的Vero细胞中进行的透射电子显微镜分析表明，WN(S)V基因组的5'端在影响质膜成熟过程中起主要作用。