Stellwagen R H, Kohli K K, Sailor R D
J Cell Physiol. 1977 Apr;91(1):51-61. doi: 10.1002/jcp.1040910106.
The increase in tyrosine aminotransferase activity which occurs in rat hepatoma tissue culture (HTC) cells in response to cyclic AMP analogs has been shown to be an enzyme induction, similar to the larger response observed in certain other hepatoma cells and in liver. A specific antibody to tyrosine aminotransferase has been used to show that the number of enzyme molecules and the rate of enzyme synthesis are increased by N6,O2'-dibutyryl cyclic AMP in HTC cells. The effect on tyrosine aminotransferase is also produced by various 8-substituted derivatives of cyclic AMP and occurs whether or not the enzyme has been preinduced with a glucocorticoid. The response of the enzyme is greater when HTC cells are maintained in monolayer than in suspension cultures. Neither cell growth nor serum is required for the response.
已证明，大鼠肝癌组织培养（HTC）细胞中，酪氨酸氨基转移酶活性因环腺苷酸类似物出现的增加是一种酶诱导现象，类似于在某些其他肝癌细胞和肝脏中 observed 的更大反应。已使用酪氨酸氨基转移酶的特异性抗体来显示，N6,O2'-二丁酰环腺苷酸可增加HTC细胞中的酶分子数量和酶合成速率。环腺苷酸的各种8-取代衍生物也会对酪氨酸氨基转移酶产生这种作用，无论该酶是否已用糖皮质激素进行过预诱导。当HTC细胞维持在单层培养时，该酶的反应比悬浮培养时更大。该反应既不需要细胞生长也不需要血清。
