Yap B K, Johnston G A, Kazlauskas R
N.S.W. Regional Laboratory, Australian Government Analytical Laboratories, Pymble.
J Chromatogr. 1992 Jan 17;573(2):183-90. doi: 10.1016/0378-4347(92)80118-a.
A method was developed for the routine screening, confirmation and quantitation of corticosteroids in human urine using bench top capillary gas chromatography (GC)-mass-selective detection. The free and conjugated corticosteroid fractions were isolated by liquid-liquid partition. After evaporation to dryness under vacuum the corticosteroid residues were derivatized to form the methyloxime trimethylsilyl ether derivatives. Both GC retention data and characteristic spectral data based on authentic reference standards were used for the identification and quantitation of cortisol, cortisone, tetrahydrocortisol and tetrahydrocortisone in the ppb (ng/ml) concentration range. The method is simpler and more efficient than the other GC-mass spectrometric (MS) techniques. It is also more sensitive than the liquid chromatographic-MS method.
开发了一种使用台式毛细管气相色谱（GC）-质量选择性检测对人尿中皮质类固醇进行常规筛查、确证和定量的方法。通过液-液分配分离游离和结合的皮质类固醇部分。在真空下蒸发至干后，将皮质类固醇残留物衍生化以形成甲基肟三甲基硅醚衍生物。基于真实参考标准的GC保留数据和特征光谱数据均用于在ppb（ng/ml）浓度范围内对皮质醇、可的松、四氢皮质醇和四氢可的松进行鉴定和定量。该方法比其他GC-质谱（MS）技术更简单、更高效。它也比液相色谱-MS方法更灵敏。
