Preece N E, Forrow S, Ghatineh S, Langley G J, Timbrell J A
Department of Biophysics, Royal College of Surgeons of England, UK.
J Chromatogr. 1992 Jan 17;573(2):227-34. doi: 10.1016/0378-4347(92)80123-8.
Plasma and liver levels of hydrazine were determined at 10, 30, 90 and 270 min in rats given 0.09, 0.27, 0.84 and 2.53 mmol of hydrazine per kg body weight orally by capillary gas chromatography-mass spectrometry of its pentafluorobenzaldehyde adduct (DFBA, m/z 388) using selected ion monitoring with 15N2-labelled hydrazine as the internal standard (adduct, m/z 390). The mean half-life for hydrazine in the plasma was approximately 2 h but varied with dose. Urinary excretion (0-24 h) of hydrazine and its metabolite acetylhydrazine were determined employing nitrogen-phosphorus detection of the adducts utilising a novel internal standard, pentafluorophenylhydrazine, the adduct of which structurally resembles DFBA. The fraction of the original dose excreted as hydrazine (and acetylhydrazine) declined with increasing dose.
通过毛细管气相色谱-质谱法，以15N2标记的肼为内标（加合物，m/z 390），对其五氟苯甲醛加合物（DFBA，m/z 388）进行选择离子监测，测定口服给予每千克体重0.09、0.27、0.84和2.53 mmol肼的大鼠在10、30、90和270分钟时的血浆和肝脏中肼的水平。血浆中肼的平均半衰期约为2小时，但随剂量而变化。采用新型内标五氟苯基肼（其加合物在结构上类似于DFBA），通过氮磷检测加合物，测定肼及其代谢物乙酰肼的尿排泄量（0 - 24小时）。以肼（和乙酰肼）形式排泄的原始剂量分数随剂量增加而下降。
