同时测定苯丙胺和N-甲基苯丙胺的电子捕获气相色谱法。

Electron-capture gas chromatographic procedure for simultaneous determination of amphetamine and N-methylamphetamine.

作者信息

Paetsch P R, Baker G B, Caffaro L E, Greenshaw A J, Rauw G A, Coutts R T

机构信息

Department of Psychiatry, University of Alberta, Edmonton, Canada.

出版信息

J Chromatogr. 1992 Jan 17;573(2):313-7. doi: 10.1016/0378-4347(92)80135-d.

DOI:10.1016/0378-4347(92)80135-d

PMID:1601966

Abstract

An electron-capture gas chromatographic procedure for the simultaneous determination of amphetamine and N-methylamphetamine in biological samples is described. The method employs extraction with the ion-pairing reagent bis(2-ethylhexyl)phosphoric acid, and back-extraction with 0.5 M hydrochloric acid. The hydrochloric acid phase is basified, and the amphetamines and the internal standard benzylamine are derivatized with pentafluorobenzenesulfonyl chloride prior to analysis on a gas chromatograph equipped with a capillary column. Levels of amphetamine and N-methylamphetamine have been determined in the urine and liver of rats treated chronically with (-)-deprenyl.

摘要

本文描述了一种用于同时测定生物样品中苯丙胺和N-甲基苯丙胺的电子捕获气相色谱法。该方法采用离子对试剂双（2-乙基己基）磷酸进行萃取，并用0.5M盐酸进行反萃取。将盐酸相碱化，在配备毛细管柱的气相色谱仪上进行分析之前，用五氟苯磺酰氯将苯丙胺类和内标苄胺衍生化。已测定长期用（-）-司来吉兰处理的大鼠尿液和肝脏中苯丙胺和N-甲基苯丙胺的含量。