Noguchi H, Yoshida K, Murano M, Naruto S
Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
J Chromatogr. 1992 Jan 17;573(2):336-8. doi: 10.1016/0378-4347(92)80140-l.
A sensitive high-performance liquid chromatographic method for a routine assay of nadolol in serum is described. Serum samples spiked with atenolol (internal standard) were extracted with diethyl ether. After centrifugation, the organic layer was evaporated to dryness. The residue was redissolved in the mobile phase and injected onto an octadecyl silica column (150 mm x 4.6 mm I.D.). The mobile phase was 0.05 M ammonium acetate (pH 4.5)-acetonitrile (85:15, v/v). Fluorometric detection (excitation 230 nm, emission 300 nm) was used. The minimum detectable level of nadolol in serum was 1 ng/ml.
描述了一种用于血清中纳多洛尔常规测定的灵敏高效液相色谱法。加入阿替洛尔（内标）的血清样品用乙醚萃取。离心后，将有机层蒸发至干。残渣重新溶解于流动相中并注入十八烷基硅胶柱（150 mm×4.6 mm内径）。流动相为0.05 M醋酸铵（pH 4.5）-乙腈（85:15，v/v）。采用荧光检测（激发波长230 nm，发射波长300 nm）。血清中纳多洛尔的最低检测水平为1 ng/ml。
