Letter W S
VAREX Corporation, Burtonsville, MD 20866.
J Chromatogr. 1992 Jan 24;590(1):169-73. doi: 10.1016/0021-9673(92)87019-5.
A preparative high-performance liquid chromatographic method, based on recycle chromatography, to separate vitamin D2 (ergocalciferol) from previtamin D is described. The method provides efficient separation by means of a mixture of methanol, acetonitrile and hexane as eluent on a reversed-phase C18 column. Scale-up to a 2-in. diameter column resulted in the collection of 100% pure fractions based on UV detection at 265 nm. The total throughput and the economics of the purification were also optimized.
描述了一种基于循环色谱的制备型高效液相色谱方法，用于从维生素原D中分离维生素D2（麦角钙化醇）。该方法通过在反相C18柱上使用甲醇、乙腈和己烷的混合物作为洗脱剂实现高效分离。放大至2英寸直径的柱后，基于265nm处的紫外检测可收集到100%的纯馏分。还对纯化的总通量和经济性进行了优化。
