Siano F, Catalfamo M, Cautela D, Servillo L, Castaldo D
Stazione Sperimentale per le Industrie delle Essenze e dei Derivati dagli Agrumi, Via Gen. Tommasini 2, I-89127 Reggio Calabria, Italy.
Food Addit Contam. 2005 Mar;22(3):197-203. doi: 10.1080/02652030500041581.
A procedure for the extraction and determination of pulegone enanthiomers in mint essential oils and mint products (syrups, dried leaves, toothpaste, lozenges, candy and chewing-gum) was developed. The compounds were recovered from the food matrices by employing a simultaneous distillation-extraction (SDE) technique with a Likens-Nickerson apparatus using dichloromethane as an extraction solvent. The analyses were performed by capillary gas chromatography mass spectrometry (GC/MS). Experiments on food products spiked at different pulegone concentrations showed recoveries ranging from 95 to 106%. The detection limit was about 5?mg?l(-1) for both pulegone enanthiomers and good linearity was found in the concentration range 0.5-25?mg?l(-1). In a number of repeated analyses, the pulegone peak height repeatability (RSD) was 0.2%. The pulegone enanthiomers were separated and quantified by enanthioselective multidimensional gas chromatography. The results of analyses conducted on essential mint oils and mint-flavoured food products are reported.
建立了一种从薄荷精油和薄荷产品（糖浆、干叶、牙膏、含片、糖果和口香糖）中提取和测定胡薄荷酮对映体的方法。采用Likens-Nickerson装置，以二氯甲烷为萃取溶剂，通过同时蒸馏萃取（SDE）技术从食品基质中回收化合物。分析采用毛细管气相色谱-质谱联用（GC/MS）法进行。对不同胡薄荷酮浓度加标的食品进行的实验表明，回收率在95%至106%之间。两种胡薄荷酮对映体的检测限约为5 mg l⁻¹，在0.5 - 25 mg l⁻¹的浓度范围内具有良好的线性关系。在多次重复分析中，胡薄荷酮峰高的重复性（RSD）为0.2%。通过对映体选择性多维气相色谱法分离并定量胡薄荷酮对映体。报告了对薄荷精油和薄荷味食品进行分析的结果。
