Nakamura Toru, Fukuda Tatsuya, Nakano Masaru, Hasebe Mitsuyasu, Kameya Toshiaki, Kanno Akira
Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, 980-8577 Aoba-ku Sendai, Japan.
Plant Mol Biol. 2005 Jun;58(3):435-45. doi: 10.1007/s11103-005-5218-z.
The class B genes, which belong to the MADS-box gene family, play important roles in regulating the development of petals and stamens in flowering plants. To understand the molecular mechanisms of floral development in Agapanthus praecox ssp. orientalis (Agapanthaceae), we isolated and characterized the homologs of the Antirrhinum majus genes GLOBOSA and DEFICIENS in this plant. These were designated as ApGLO and ApDEF, respectively. ApGLO and ApDEF contain open reading frames that encode deduced protein with 210 and 214 amino acid residues, respectively. Phylogenetic analysis indicated that ApGLO and ApDEF belong to the monocot class B gene family. In situ hybridization experiments revealed that hybridization signals of ApGLO and ApDEF were observed in whorl 1 as well as in whorls 2 and 3. Moreover, the flowers of transgenic Arabidopsis plants that ectopically expressed ApGLO formed petal-like organs in whorl 1. These observations indicate that the flower developmental mechanism of Agapanthus follows the modified ABC model.
B类基因属于MADS盒基因家族，在调控开花植物花瓣和雄蕊的发育中发挥重要作用。为了解东方百子莲（百子莲科）花发育的分子机制，我们分离并鉴定了该植物中金鱼草基因GLOBOSA和DEFICIENS的同源基因。它们分别被命名为ApGLO和ApDEF。ApGLO和ApDEF包含开放阅读框，分别编码推导的含有210和214个氨基酸残基的蛋白质。系统发育分析表明，ApGLO和ApDEF属于单子叶植物B类基因家族。原位杂交实验显示，在第1轮以及第2和第3轮中观察到ApGLO和ApDEF的杂交信号。此外，异位表达ApGLO的转基因拟南芥植物的花在第1轮中形成了花瓣状器官。这些观察结果表明，百子莲的花发育机制遵循改良的ABC模型。
