Zagdańska Renata, Grzelewska-Rzymowska Iwona, Górski Paweł, Kuźmińska Beata
Kliniki Gruźlicy, Chorób i Nowotworów Płuc Lódź.
Pneumonol Alergol Pol. 2004;72(9-10):403-8.
Asthma is a chronic inflammatory disease of the airways in which many cell types play a role. Although the most important cells are eosinophils, there are suggestions that also neutrophils may play a role in asthma. The aim of the study was to measure and compare chemotactic activity of neutrophils in patients with severe asthma and with COPD. We examined 49 patients with severe asthma and 23 patients with COPD. The mean number of neutrophils in peripheral blood of 20 asthmatics with irreversible airflow obstruction was 3.96 x 10(6)/ml. The chemotactic activity of neutrophils to FMLP was 2.69 SEM +/- 0.4, to IL-8 in concentration 10-7 microg/ml - 1.64, SEM +/- 0.2, and to IL-8 in concentration 10-8 microg/ml - 1.17, SEM +/- 0.1. The mean number of neutrophils in 29 asthmatics with reversible airflow obstruction was 3.08 x 10(6)/ml. Their chemotactic activity to fMLP was 1.7, SEM +/- 0.1 to IL-8 in concentration 10-7 microg/ml - 1.51. SEM +/- 0.2, and to IL-8 in concentration 10-8 microg/ml - 1.08, SEM +/- 0.1. The mean number of neutrophils in COPD patients was 4.05 x 10(6)/ml and their chemotactic activity to FMLP was 1.9, SEM +/- 0.1 to IL-8 - 1.35, SEM +/- 0.1. All asthmatic patients were treated with inhaled corticosteroids and some of them with oral corticosteroids. Despite of that treatment the number of neutrophils isolated from patients with asthma with irreversible airflow obstruction was almost the same like in COPD patients and chemotactic activity of neutrophils in this group was the highest. We concluded that corticosteroids treatment did not diminished chemotactic activity of neutrophils isolated from patient suffering from asthma with irreversible airflow obstruction.
哮喘是一种气道慢性炎症性疾病，多种细胞类型参与其中。虽然最重要的细胞是嗜酸性粒细胞，但有迹象表明中性粒细胞在哮喘中也可能发挥作用。本研究的目的是测量和比较重度哮喘患者和慢性阻塞性肺疾病（COPD）患者中性粒细胞的趋化活性。我们检查了49例重度哮喘患者和23例COPD患者。20例存在不可逆气流受限的哮喘患者外周血中性粒细胞的平均数量为3.96×10⁶/ml。中性粒细胞对N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（FMLP）的趋化活性为2.69标准误±0.4，对浓度为10⁻⁷μg/ml的白细胞介素-8（IL-8）的趋化活性为1.64，标准误±0.2，对浓度为10⁻⁸μg/ml的IL-8的趋化活性为1.17，标准误±0.1。29例存在可逆气流受限的哮喘患者中性粒细胞的平均数量为3.08×10⁶/ml。他们对fMLP的趋化活性为1.7，标准误±0.1，对浓度为10⁻⁷μg/ml的IL-8的趋化活性为1.51，标准误±0.2，对浓度为10⁻⁸μg/ml的IL-8的趋化活性为1.08，标准误±0.1。COPD患者中性粒细胞的平均数量为4.05×10⁶/ml，他们对FMLP的趋化活性为1.9，标准误±0.1，对IL-8的趋化活性为1.35，标准误±0.1。所有哮喘患者均接受吸入性糖皮质激素治疗，部分患者还接受口服糖皮质激素治疗。尽管进行了这种治疗，但从存在不可逆气流受限的哮喘患者中分离出的中性粒细胞数量与COPD患者几乎相同，且该组中性粒细胞的趋化活性最高。我们得出结论，糖皮质激素治疗并未降低从存在不可逆气流受限的哮喘患者中分离出的中性粒细胞的趋化活性。
