Bartling Babett, Hofmann Hans-Stefan, Boettger Thomas, Hansen Gesine, Burdach Stefan, Silber Rolf-Edgar, Simm Andreas
Department of Cardio-Thoracic Surgery, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany.
Lung Cancer. 2005 Aug;49(2):145-54. doi: 10.1016/j.lungcan.2005.02.006.
Expression profiling by gene microarray techniques have been developed to predict malignant tissue but there are no experiences with the application of antibody arrays to identify malignancy-related proteins. Because altered protein patterns might also better interpret biological processes, we applied tumour samples from 12 patients with squamous cell lung carcinoma and individual lung tissue controls to antibody arrays spotted with 378 distinct monoclonal antibodies. Array analysis defined 20 proteins with higher and nine with lower abundance in lung tumours. Comparison with gene microarray data revealed that 31% of the differentially regulated proteins correlate with altered mRNA expression in squamous cell lung carcinomas, including PEX1, MKK7 and HDAC3 for up-regulated proteins. The histone deacetylase (HDAC) 3 was investigated in detail by immunoblot analysis showing that HDAC3 is indeed elevated in 92% of tumours (n=22/24; P<0.001). Thus, antibody microarrays can be useful for detection of some target proteins related to lung cancer.
基因微阵列技术的表达谱分析已被用于预测恶性组织，但在应用抗体阵列识别恶性肿瘤相关蛋白方面尚无经验。由于蛋白质模式的改变可能也能更好地解释生物学过程，我们将12例肺鳞状细胞癌患者的肿瘤样本及个体肺组织对照应用于点有378种不同单克隆抗体的抗体阵列。阵列分析确定了肺肿瘤中丰度较高的20种蛋白和丰度较低的9种蛋白。与基因微阵列数据比较显示，31%差异调节的蛋白与肺鳞状细胞癌中mRNA表达的改变相关，上调蛋白包括PEX1、MKK7和HDAC3。通过免疫印迹分析对组蛋白去乙酰化酶（HDAC）3进行了详细研究，结果显示92%的肿瘤（n = 22/24；P < 0.001）中HDAC3确实升高。因此，抗体微阵列可用于检测一些与肺癌相关的靶蛋白。
