Amodu O K, Adeyemo A A, Ayoola O O, Gbadegesin R A, Orimadegun A E, Akinsola A K, Olumese P E, Omotade O O
College of Medicine, Institute of Child Health, University of Ibadan, Ibadan, Nigeria.
Acta Trop. 2005 Sep;95(3):226-32. doi: 10.1016/j.actatropica.2005.06.017.
Genetic characteristics of Plasmodium falciparum may play a role in the clinical severity of malaria infection. We have studied the association between diversity at the merozoite surface protein-1 (msp-1) locus and the severity of disease in childhood malaria in Ibadan, south-west Nigeria. Two hundred and twenty-three children (median age of 34.5 months) presenting with malaria were enrolled into the study. They comprised 53 children with asymptomatic malaria (ASM), 101 with acute uncomplicated malaria (UM) and 69 with severe malaria (SM). Genotyping of the msp-1 locus was by polymerase chain reaction. The distribution of msp-1 alleles was significantly different between the three groups. Asymptomatic malaria samples had a higher median number of alleles than the other two groups. The type of msp-1 allele detected was significantly associated with the clinical category of malaria. The absence of K1 alleles was associated with a three-fold increase risk of UM and a four-fold increased risk of SM when compared with asymptomatic malaria. The absence of MAD20 alleles was associated with a five-fold increase risk of UM and an eight-fold increase of SM. We have found an association between the msp-1 locus of P. falciparum and clinical severity of malaria in a sample of Nigerian children. Our findings show that the presence of the K1 and MAD20 alleles was significantly associated with ASM and consequently a reduced risk of developing the symptomatic disease.
恶性疟原虫的遗传特征可能在疟疾感染的临床严重程度中起作用。我们研究了裂殖子表面蛋白-1（msp-1）基因座的多样性与尼日利亚西南部伊巴丹儿童疟疾疾病严重程度之间的关联。223名患疟疾的儿童（中位年龄34.5个月）被纳入研究。他们包括53名无症状疟疾（ASM）儿童、101名急性非复杂性疟疾（UM）儿童和69名重症疟疾（SM）儿童。msp-1基因座的基因分型采用聚合酶链反应。三组之间msp-1等位基因的分布有显著差异。无症状疟疾样本的等位基因中位数高于其他两组。检测到的msp-1等位基因类型与疟疾的临床分类显著相关。与无症状疟疾相比，K1等位基因缺失与UM风险增加三倍和SM风险增加四倍相关。MAD20等位基因缺失与UM风险增加五倍和SM风险增加八倍相关。我们在一组尼日利亚儿童样本中发现了恶性疟原虫msp-1基因座与疟疾临床严重程度之间的关联。我们的研究结果表明，K1和MAD20等位基因的存在与ASM显著相关，因此出现症状性疾病的风险降低。
