前铁调素定位于肝细胞中的高尔基体区室和分泌途径。
Prohepcidin localises to the Golgi compartment and secretory pathway in hepatocytes.
作者信息
Wallace Daniel F, Summerville Lesa, Lusby Patricia E, Subramaniam V Nathan
机构信息
Membrane Transport Laboratory, The Queensland Institute of Medical Research, 300 Herston Road, Herston, Brisbane, QLD 4006, Australia.
出版信息
J Hepatol. 2005 Oct;43(4):720-8. doi: 10.1016/j.jhep.2005.02.047.
BACKGROUND/AIMS: Hepcidin is a liver-expressed peptide which plays an important role in the regulation of iron metabolism. It is a negative regulator of iron absorption and release of iron from cells. The aims of this study were to analyse the expression and localisation of prohepcidin in liver and cell lines.
METHODS
We generated antibodies against recombinant mouse prohepcidin and studied its expression in cell lines, primary hepatocytes and livers of normal mice and mice with abnormalities in iron metabolism.
RESULTS
Prohepcidin localised to the secretory pathway, primarily the Golgi apparatus in liver cells and tissues. Hfe and beta2-microglobulin knockout mice have similar levels of prohepcidin protein expression as compared to wild-type mice despite increased iron stores. Sex-linked anaemia mice have iron deficiency and no prohepcidin expression in the liver.
CONCLUSIONS
Prohepcidin protein is present in the secretory pathway of liver cells. Despite iron loading, mouse models of haemochromatosis have comparatively normal levels of prohepcidin expression whereas mice with iron deficiency have no prohepcidin expression.
背景/目的：铁调素是一种在肝脏表达的肽，在铁代谢调节中起重要作用。它是铁吸收和细胞内铁释放的负调节因子。本研究的目的是分析肝脏和细胞系中前铁调素的表达及定位。
方法
我们制备了针对重组小鼠前铁调素的抗体，并研究了其在细胞系、原代肝细胞以及正常小鼠和铁代谢异常小鼠肝脏中的表达。
结果
前铁调素定位于分泌途径，主要在肝细胞和组织的高尔基体中。尽管铁储存增加，但与野生型小鼠相比，遗传性血色素沉着症基因（Hfe）和β2-微球蛋白基因敲除小鼠的前铁调素蛋白表达水平相似。X连锁贫血小鼠存在缺铁情况，肝脏中无铁调素表达。
结论
前铁调素蛋白存在于肝细胞的分泌途径中。尽管有铁过载，但血色素沉着症小鼠模型的前铁调素表达水平相对正常，而缺铁小鼠则无铁调素表达。
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