一株新的嗜温栖热放线菌菌株IFP 2015在甲基叔丁基醚上生长的分离与鉴定
Isolation and characterization of a new Mycobacterium austroafricanum strain, IFP 2015, growing on MTBE.
作者信息
Lopes Ferreira Nicolas, Maciel Helena, Mathis Hugues, Monot Frédéric, Fayolle-Guichard Françoise, Greer Charles W
机构信息
Institut Français du Pétrole, 1-4, avenue de Bois-Préau, 92852, Rueil-Malmaison, France.
出版信息
Appl Microbiol Biotechnol. 2006 Apr;70(3):358-65. doi: 10.1007/s00253-005-0074-y. Epub 2005 Jul 19.
A new Mycobacterium austroafricanum strain, IFP 2015, growing on methyl tert-butyl ether (MTBE) as a sole carbon source was isolated from an MTBE-degrading microcosm inoculated with drain water of an MTBE-supplemented gasoline storage tank. M. austroafricanum IFP 2015 was able to grow on tert-butyl formate, tert-butyl alcohol (TBA) and alpha-hydroxyisobutyrate. 2-Methyl-1,2-propanediol was identified as the TBA oxidation product in M. austroafricanum IFP 2015 and in the previously isolated M. austroafricanum IFP 2012. M. austroafricanum IFP 2015 also degraded ethyl tert-butyl ether more rapidly than M. austroafricanum IFP 2012. Specific primers designed to monitor the presence of M. austroafricanum strains could be used as molecular tools to detect similar strains in MTBE-contaminated environment.
从接种了添加甲基叔丁基醚（MTBE）的汽油储存罐排水的MTBE降解微观世界中分离出一种新的非洲分枝杆菌菌株IFP 2015，它能以MTBE作为唯一碳源生长。非洲分枝杆菌IFP 2015能够在甲酸叔丁酯、叔丁醇（TBA）和α-羟基异丁酸上生长。在非洲分枝杆菌IFP 2015和先前分离出的非洲分枝杆菌IFP 2012中，2-甲基-1,2-丙二醇被鉴定为TBA氧化产物。与非洲分枝杆菌IFP 2012相比，非洲分枝杆菌IFP 2015对乙基叔丁基醚的降解速度也更快。设计用于监测非洲分枝杆菌菌株存在的特异性引物可作为分子工具，用于检测MTBE污染环境中的类似菌株。
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