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由一种过表达基因组衍生的奈瑟氏菌抗原1870的脑膜炎球菌外膜囊泡疫苗引发的保护性抗体反应。

Protective antibody responses elicited by a meningococcal outer membrane vesicle vaccine with overexpressed genome-derived neisserial antigen 1870.

作者信息

Hou Victor C, Koeberling Oliver, Welsch Jo Anne, Granoff Dan M

机构信息

Children's Hospital Oakland Research Institute, Oakland, California 94609, USA.

出版信息

J Infect Dis. 2005 Aug 15;192(4):580-90. doi: 10.1086/432102. Epub 2005 Jul 15.

DOI:10.1086/432102
PMID:16028126
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2373260/
Abstract

Background. Meningococcal outer membrane vesicle (OMV) vaccines are efficacious in humans but have serosubtype-specific serum bactericidal antibody responses directed at the porin protein PorA and the potential for immune selection of PorA-escape mutants.Methods. We prepared an OMV vaccine from a Neisseria meningitidis strain engineered to overexpress genome-derived neisserial antigen (GNA) 1870, a lipoprotein discovered by genome mining that is being investigated for use in a vaccine.Results. Mice immunized with the modified GNA1870-OMV vaccine developed broader serum bactericidal antibody responses than control mice immunized with a recombinant GNA1870 protein vaccine or an OMV vaccine prepared from wild-type N. meningitidis or a combination of vaccines prepared from wild-type N. meningitidis and recombinant protein. Antiserum from mice immunized with the modified GNA1870-OMV vaccine also elicited greater deposition of human C3 complement on the surface of live N. meningitidis bacteria and greater passive protective activity against meningococcal bacteremia in infant rats. A N. meningitidis mutant with decreased expression of PorA was more susceptible to bactericidal activity of anti-GNA1870 antibodies.Conclusions. The modified GNA1870-OMV vaccine elicits broader protection against meningococcal disease than recombinant GNA1870 protein or conventional OMV vaccines and also has less risk of selection of PorA-escape mutants than a conventional OMV vaccine.

摘要

背景。脑膜炎球菌外膜囊泡（OMV）疫苗对人类有效，但针对孔蛋白PorA会产生血清亚型特异性血清杀菌抗体反应，且存在PorA逃逸突变体的免疫选择可能性。

方法。我们从一株经基因工程改造过的脑膜炎奈瑟菌菌株制备了一种OMV疫苗，该菌株可过表达基因组衍生的奈瑟菌抗原（GNA）1870，这是一种通过基因组挖掘发现的脂蛋白，正在作为疫苗进行研究。

结果。用改良的GNA1870 - OMV疫苗免疫的小鼠比用重组GNA1870蛋白疫苗、野生型脑膜炎奈瑟菌制备的OMV疫苗或野生型脑膜炎奈瑟菌与重组蛋白制备的疫苗组合免疫的对照小鼠产生了更广泛的血清杀菌抗体反应。用改良的GNA1870 - OMV疫苗免疫的小鼠的抗血清在活的脑膜炎奈瑟菌表面也引发了更多的人C3补体沉积，并对幼鼠的脑膜炎球菌菌血症具有更强的被动保护活性。一株PorA表达降低的脑膜炎奈瑟菌突变体对抗GNA1870抗体的杀菌活性更敏感。

结论。改良的GNA1870 - OMV疫苗比重组GNA1870蛋白或传统OMV疫苗能引发更广泛的针对脑膜炎球菌病的保护作用，并且与传统OMV疫苗相比，选择PorA逃逸突变体的风险也更低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/0b9c3043e26c/nihms39813f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/f3a6b5b3b439/nihms39813f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/0790d87c6d18/nihms39813f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/5766a0497ccb/nihms39813f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/0b9c3043e26c/nihms39813f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/f3a6b5b3b439/nihms39813f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/9050c2d5e07d/nihms39813f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/dc53ed5107e6/nihms39813f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/88f1cf2beb6c/nihms39813f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/0790d87c6d18/nihms39813f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/5766a0497ccb/nihms39813f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c49/2373260/0b9c3043e26c/nihms39813f7.jpg

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本文引用的文献

1
The region comprising amino acids 100 to 255 of Neisseria meningitidis lipoprotein GNA 1870 elicits bactericidal antibodies.包含脑膜炎奈瑟菌脂蛋白GNA 1870第100至255位氨基酸的区域可引发杀菌抗体。
Infect Immun. 2005 Feb;73(2):1151-60. doi: 10.1128/IAI.73.2.1151-1160.2005.
2
Expression of heterologous antigens in commensal Neisseria spp.: preservation of conformational epitopes with vaccine potential.共生奈瑟菌属中异源抗原的表达：具有疫苗潜力的构象表位的保留。
Infect Immun. 2004 Nov;72(11):6511-8. doi: 10.1128/IAI.72.11.6511-6518.2004.
3
Prevention of group B meningococcal disease by vaccination: a difficult task.通过接种疫苗预防B群脑膜炎球菌病：一项艰巨的任务。
N Z Med J. 2004 Aug 20;117(1200):U1016.
4
Getting to grips with an epidemic.应对一场流行病。
Nurs N Z. 2004 Aug;10(7):2.
5
PorA variable regions of Neisseria meningitidis.脑膜炎奈瑟菌的PorA可变区
Emerg Infect Dis. 2004 Apr;10(4):674-8. doi: 10.3201/eid1004.030247.
6
Antigenic and/or phase variation of PorA protein in non-subtypable Neisseria meningitidis strains isolated in Spain.在西班牙分离出的不可分型脑膜炎奈瑟菌菌株中PorA蛋白的抗原性和/或相变异
J Med Microbiol. 2004 Jun;53(Pt 6):515-518. doi: 10.1099/jmm.0.05517-0.
7
Protective activity of monoclonal antibodies to genome-derived neisserial antigen 1870, a Neisseria meningitidis candidate vaccine.针对基因组衍生的奈瑟氏菌抗原1870的单克隆抗体的保护活性，一种脑膜炎奈瑟氏菌候选疫苗
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8
Antibody to genome-derived neisserial antigen 2132, a Neisseria meningitidis candidate vaccine, confers protection against bacteremia in the absence of complement-mediated bactericidal activity.针对基因组衍生的奈瑟氏菌抗原2132（一种脑膜炎奈瑟氏菌候选疫苗）的抗体，在缺乏补体介导的杀菌活性的情况下可提供针对菌血症的保护。
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9
Conformational epitopes recognized by protective anti-neisserial surface protein A antibodies.被保护性抗奈瑟氏菌表面蛋白A抗体识别的构象表位。
Infect Immun. 2003 Dec;71(12):6844-9. doi: 10.1128/IAI.71.12.6844-6849.2003.
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J Exp Med. 2003 Mar 17;197(6):789-99. doi: 10.1084/jem.20021911.