Gibson G E, Toral-Barza L
Cornell University Medical College, Burke Medical Research Institute, White Plains, NY 10605.
Mech Ageing Dev. 1992 Mar 15;63(1):1-9. doi: 10.1016/0047-6374(92)90012-3.
Altered cellular calcium homeostasis may be important in the pathophysiology of aging and Alzheimer's disease. Calcium transport by freshly isolated lymphocytes declines with Alzheimer's disease compared with age-matched controls. To determine if these changes occur in the absence of complications due to drugs, diet or any of the other variables that are dependent upon the state of the patients, cytosolic free calcium ([Ca2+]i) was determined in cultured lymphoblasts from young and aged control subjects, as well as from Alzheimer patients. Lymphoblast [Ca2+]i was determined with the fluorescent probe fura-2 in either the presence or absence of serum. In cells that were grown in serum free medium, neither growth rates nor [Ca2+]i varied between groups. Growing cells in serum containing medium doubled growth rates and [Ca2+]i. However, [Ca2+]i from young, aged and Alzheimer groups were still similar. Thus, an age- or Alzheimer-related alteration in [Ca2+]i does not occur in cultured lymphoblasts.
细胞钙稳态的改变可能在衰老和阿尔茨海默病的病理生理学中起重要作用。与年龄匹配的对照组相比，新鲜分离的淋巴细胞的钙转运在阿尔茨海默病患者中有所下降。为了确定这些变化是否在不存在药物、饮食或任何其他取决于患者状态的变量所引起的并发症的情况下发生，我们测定了来自年轻和老年对照受试者以及阿尔茨海默病患者的培养成淋巴细胞中的胞质游离钙（[Ca2+]i）。在有或无血清的情况下，用荧光探针fura-2测定成淋巴细胞的[Ca2+]i。在无血清培养基中生长的细胞，各组之间的生长速率和[Ca2+]i均无差异。在含血清培养基中生长的细胞，生长速率和[Ca2+]i增加一倍。然而，年轻、老年和阿尔茨海默病组的[Ca2+]i仍然相似。因此，在培养的成淋巴细胞中未发生与年龄或阿尔茨海默病相关的[Ca2+]i改变。
