用于蛋白质固定的人工多肽支架。
Artificial polypeptide scaffold for protein immobilization.
作者信息
Zhang Kechun, Diehl Michael R, Tirrell David A
机构信息
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
出版信息
J Am Chem Soc. 2005 Jul 27;127(29):10136-7. doi: 10.1021/ja051457h.
An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl-tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.
设计了一种由表面锚定结构域和蛋白质捕获结构域组成的人工多肽支架，并在体内进行表达。通过使用突变的大肠杆菌苯丙氨酰 - tRNA合成酶，将光反应性氨基酸对叠氮基苯丙氨酸掺入表面锚定结构域。通过旋涂和光交联，用该多肽对经辛基三氯硅烷处理的表面进行功能化。结果表明，所得蛋白质膜通过卷曲螺旋异二聚体的缔合固定重组蛋白。
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