Schaller M, Loewenstein M, Borelli C, Jacob K, Vogeser M, Burgdorf W H C, Plewig G
Department of Dermatology and Allergology, University of Munich, Germany.
Br J Dermatol. 2005 Jul;153(1):66-71. doi: 10.1111/j.1365-2133.2005.06530.x.
The inflammation in acne vulgaris is widely thought to be induced by an immunological reaction, but the role of Propionibacterium acnes is unclear.
To examine the local host response mechanism of a keratinocyte cell line 3 h and 6 h after stimulation with viable and heat-killed P. acnes.
The quantitative expression of cytokines was measured at the mRNA level by real-time reverse transcription-polymerase chain reaction.
The coincubation of a keratinocyte cell line with viable, but not heat-killed, P. acnes modulated an adequate cytokine response for interleukin (IL)-1beta, granulocyte/macrophage colony-stimulating factor and IL-8. High-performance liquid chromatographic analysis of the in vivo porphyrin pattern secreted by P. acnes revealed a predominance of coproporphyrin III in acne lesions. This same porphyrin fraction also modestly induced IL-8 expression by keratinocytes.
This cytokine pattern may favour a chemotactic response and implicates P. acnes and coproporphyrin III in the recruitment of inflammatory cells to the site of infection and in the development of acne lesions.
寻常痤疮中的炎症普遍被认为是由免疫反应诱导的，但痤疮丙酸杆菌的作用尚不清楚。
研究角质形成细胞系在活的和热灭活的痤疮丙酸杆菌刺激后3小时和6小时的局部宿主反应机制。
通过实时逆转录聚合酶链反应在mRNA水平测量细胞因子的定量表达。
角质形成细胞系与活的而非热灭活的痤疮丙酸杆菌共同孵育，可调节白细胞介素（IL）-1β、粒细胞/巨噬细胞集落刺激因子和IL-8的适当细胞因子反应。对痤疮丙酸杆菌分泌的体内卟啉模式进行高效液相色谱分析，结果显示痤疮皮损中粪卟啉III占主导。相同的卟啉部分也适度诱导角质形成细胞表达IL-8。
这种细胞因子模式可能有利于趋化反应，并表明痤疮丙酸杆菌和粪卟啉III在将炎症细胞募集到感染部位以及痤疮皮损的形成中起作用。
