Spanheimer R G
Department of Medicine, University of Iowa, Iowa City 52246.
Matrix. 1992 Apr;12(2):101-7. doi: 10.1016/s0934-8832(11)80051-x.
Collagen production has been shown to be decreased in costal cartilage from nondiabetic animals after incubation with diabetic rat serum. Since collagen was decreased to a similar degree in tissues from diabetic animals, we questioned whether altered collagen production in vivo could be related to altered production induced in vitro. Collagen and noncollagen protein production in articular cartilage from diabetic animals (production in vivo) was compared to protein production in dermal fibroblasts from non-diabetic rats exposed to serum from the same diabetic rats (production in vitro). Diabetes was induced by intravenous administration of 90 mg/kg of streptozotocin into male Sprague-Dawley rats. Cartilage was removed and incubated with [3H]-proline for 2 hours at 37 degrees C (in vivo), while fibroblasts were exposed to experimental serum from individual animals for 24 hours with addition of 5 microCi of [5-3H]-proline for the final 6 hours (in vitro). Collagen and noncollagen protein production were quantitated using purified bacterial collagenase. Collagen production in cartilage decreased to 46% (p less than .01) and noncollagen to 68% (p less than .05) of levels in control animals. Fibroblasts exposed to 2.5% diabetic serum decreased collagen and noncollagen protein production to levels of 30% (p less than .01) and 54% (p less than .05) of production in cells incubated in 2.5% normal rat serum. Correlation between defective collagen production in cartilage from individual rats and the effects of their own serum on collagen production in fibroblasts was significant (r = 0.84, p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
已表明，用糖尿病大鼠血清孵育后，非糖尿病动物肋软骨中的胶原蛋白生成减少。由于糖尿病动物组织中的胶原蛋白减少程度相似，我们质疑体内胶原蛋白生成的改变是否可能与体外诱导的生成改变有关。将糖尿病动物关节软骨中的胶原蛋白和非胶原蛋白生成（体内生成）与暴露于相同糖尿病大鼠血清的非糖尿病大鼠皮肤成纤维细胞中的蛋白质生成（体外生成）进行比较。通过向雄性Sprague-Dawley大鼠静脉注射90 mg/kg链脲佐菌素诱导糖尿病。取出软骨并在37℃下与[3H]-脯氨酸孵育2小时（体内），而成纤维细胞则暴露于来自个体动物的实验血清中24小时，并在最后6小时添加5 μCi的[5-3H]-脯氨酸（体外）。使用纯化的细菌胶原酶对胶原蛋白和非胶原蛋白生成进行定量。软骨中的胶原蛋白生成降至对照动物水平的46%（p<0.01），非胶原蛋白降至68%（p<0.05）。暴露于2.5%糖尿病血清的成纤维细胞将胶原蛋白和非胶原蛋白生成降至在2.5%正常大鼠血清中孵育的细胞生成水平的30%（p<0.01）和54%（p<0.05）。个体大鼠软骨中胶原蛋白生成缺陷与其自身血清对成纤维细胞中胶原蛋白生成的影响之间的相关性显著（r = 0.84，p<0.001）。（摘要截断于250字）
