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Activation and quantitative estimation of bacteriophage T4 late regulatory signal in cis- and transconditions.

作者信息

Noguchi T, Takahashi H

机构信息

Research Laboratories, Yamasa Shoyu Co. Ltd., Chiba, Japan.

出版信息

Mol Gen Genet. 1992 May;233(1-2):319-21. doi: 10.1007/BF00587595.

DOI:10.1007/BF00587595
PMID:1603073
Abstract

Activation of the bacteriophage T4 late gene 22 carried on the phage genome (ciscondition) or borne on a plasmid (transcondition) was quantitatively analyzed after phage infection using a lacZ reporter gene. Transcription from the late promoter was activated postreplicatively by concomitantly infecting T4 phage. A truncated T4-lacZ gene including only 15 bp of sequence upstream of the late promoter consensus of gene 22 TATAAATA, was fully activated in the ciscondition but induced to a reduced extent in the transcondition. A possible mechanism for trans-activation is discussed.

摘要

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引用本文的文献

1
Transactivation of a plasmid-borne bacteriophage T4 late gene.质粒携带的噬菌体T4晚期基因的反式激活
Mol Gen Genet. 1993 Jun;239(3):393-9. doi: 10.1007/BF00276937.

本文引用的文献

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An efficient method for constructing a chimeric bacteriophage T4 to estimate the regulatory signals.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):620-7. doi: 10.1016/0006-291x(91)91417-b.
9
Utilization of trans-activated T4 uvsY regulatory signal for a high yield of cloned gene products.利用反式激活的T4 uvsY调控信号实现克隆基因产物的高产。
Agric Biol Chem. 1991 Oct;55(10):2497-506.
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Design of a system of conditional lethal mutations (tab/k/com) affecting protein-protein interactions in bacteriophage T4-infected Escherichia coli.
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